Characterization of β-1，4-mannanase from Bacillus pumilus and heterologous expression in Lactobacillus casei

Abstract:

Abstract:［Objective］Lactobacillus casei is widely used in food production and feed industry. The aim of this study was to construct the recombinant expression mannanase Lb. casei.［Methods］The mature peptide gene of β-1，4-mannanase from Bacillus pumilus was cloned into expression vectors pELX1 and pELSH，then electroporated into Lb．casei，establishing an intracellular and a secretion expression mannanase Lb． casei respectively.［Results］After incubation，the specific activity of β-1，4-mannanase was 23 U/mg whole cell protein for intracellular expression and 8.8 U/mL forsecretion expression in supernatant.［Conclusion］Mannanase gene expression in Lb．casei provides application prospect and deserves further study．

Key words:Keywords: β-1，4-mannanase，enzyme property，Lactobacillus casei，intracellular/secretion expression