Lijuan Hong
Department of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou 450001, Henan Province, ChinaBing Zhang
Department of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou 450001, Henan Province, ChinaGuangcai Duan
Department of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou 450001, Henan Province, China;Henan Innovation Center of Molecular Diagnosis and Laboratory Medicine, Xinxiang Medical University, Xinxiang 453003, Henan Province, ChinaWenjuan Liang
Department of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou 450001, Henan Province, China;Henan Innovation Center of Molecular Diagnosis and Laboratory Medicine, Xinxiang Medical University, Xinxiang 453003, Henan Province, ChinaYingfang Wang
Department of Preventive Medicine, Henan University of Science and Technology, Luoyang 471003, Henan Province, ChinaShuaiyin Chen
Department of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou 450001, Henan Province, ChinaHaiyan Yang
Department of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou 450001, Henan Province, ChinaYuanlin Xi
Department of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou 450001, Henan Province, China[Objective] To analyze the relationship between CRISPR/Cas system and drug-resistance, virulence. To investigate the effect of IS600 on the expression of CRISPR associated gene cse2 in Shigella.[Methods] CRISPR loci, CRISPR associated gene cse2, drug-resistant genes and virulent genes were detected by PCR in 33 Shigella strains; Trypan Blue counting test was used to detect bacterial virulence; Real-time PCR was used to detect relative mRNA expression of cse2; susceptibilities of Shigella strains were tested by agar diffusion method. Furthermore, we analyzed the relationship between CRISPR loci and drug-resistant genes, virulent genes. The effect of the IS600 on the expression of CRISPR associated gene cse2 was investigated.[Results] The mortality of Hela cells infected by Shigella with CRISPR1 loci was significantly lower (P<0.05) than those infected by Shigella without CRISPR1. The mRNA expression level of cse2 in group with IS600 was significantly (P<0.05) lower than that in group without IS600.[Conclusions] CRISPR loci were widely present in Shigella. Shigella without CRISPR1 has a higher pathogenicity. Due to the insertion of IS600, the mRNA expression level of cse2 was decreased in Shigella.
Lijuan Hong, Bing Zhang, Guangcai Duan, Wenjuan Liang, Yingfang Wang, Shuaiyin Chen, Haiyan Yang, Yuanlin Xi. Role of CRISPR/Cas systems in drugresistance and virulence and the effect of IS600 on the expression of cse2 in Shigella. [J]. Acta Microbiologica Sinica, 2016, 56(12): 1912-1923
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