Home > Archive>Volume 57, Issue 2, 2017 >240-253. DOI:10.13343/j.cnki.wsxb.20160247
PDF HTML XML Export Cite reminder
Physicochemical property and antioxidant activity of exopolysaccharide produced by endophytic fungal Fusarium redolens 6WBY3 isolated from Fritillaria unibracteata var. wabuensis
Author:
  • Article
    • | |
  • Metrics
    • |
  • Reference [27]
    • |
  • Related [20]
    • | | |
  • Comments
    Abstract:

    [Objective] To study the exopolysaccharide (EPS) of endophytic fungal Fusarium redolens 6WBY3 isolated from Fritillaria unibracteata var. wabuensis (FUW) including its antioxidant activities.[Methods] We isolated the EPS from the culture medium of strain 6WBY3 using the methods of degreasing with organic solvent, precipitation with ethanol, decoloration with macroporous resins, deproteinization with protease in combination with sevag reagent, desalination with dialysis and separation with DEAE-cellulose ion exchange chromatography. Then, we analyzed the EPS fractions using high-performance gel-permeation chromatography with evaporative light scattering detector (HPGPC-ELSD), ultra violet (UV) spectra, scanning electron microscope (SEM), PMP precolumn derivatization with high performance liquid chromatography (PMP-HPLC), fourier transform infrared (FT-IR) spectroscopy and 1H-nuclear magnetic resonance (1H NMR) methods. We evaluated the antioxidant activity of the EPS using DPPH and ABTS radical scavenging activities and iron ion chelating ability methods.[Results] We obtained two homogeneous EPSs, namely 6WBY3EPS-3 and 6WBY3EPS-4, with the molecular weight (Mw) of 17.41×106 and 8.84×105 Da, respectively. 6WBY3EPS-3 was composed of mannose, glucose and galactose in a molar ratio of 8.16:4.96:10.00, while 6WBY3EPS-4 was composed of mannose, rhamnose, glucose and galactose in a molar ratio of 8.08:1.71:6.32:10.00. The results of SEM showed that 6WBY3EPS-3 was the irregular multilateral body, and 6WBY3EPS-4 was rules of quadrilateral body. The results of FT-IR and 1H NMR analysis exhibited that 6WBY3EPS-3 was acidic polysaccharose with abundant galactofuranose, mannofuranose and a few α-D-glucopyranose, while 6WBY3EPS-4 possessed pyranose ring mainly. Those two EPSs had anomeric hydrogen with α-(main) and β-glycosidic configuration. Furthermore, the results of antioxidant activity suggested that both 6WBY3EPS-3 and 6WBY3EPS-4 had a weak DPPH radical scavenging ability, a moderate ABTS radical scavenging activity and a moderate iron ion chelating effect.[Conclusion] The two EPSs from the endophytic fungus 6WBY3 were firstly obtained and investigated. Our investigation indicated that the EPS from 6WBY3 had the application potential as an antioxidant in medicine and food industries. In addition, this work can also provide theory reference for improving development of EPS from other endophytic fungi.

    Reference
    [1] Meng L, Zhang LW. Immune regulation activity and mechanism of Tibetan Kefir exopolysaccharide fractions. Acta Microbiologica Sinica, 2009, 49(12):1660-1664. (in Chinese)孟利, 张兰威. 西藏灵菇胞外多糖组分对小鼠免疫调节作用及机制的研究. 微生物学报, 2009, 49(12):1660-1664.
    [2] Chen Y, Mao WJ, Yang YP, Teng XC, Zhu WM, Qi XH, Chen YL, Zhao CQ, Hou YJ, Wang CY, Li N. Structure and antioxidant activity of an extracellular polysaccharide from coral-associated fungus, Aspergillus versicolor LCJ-5-4. Carbohydrate Polymers, 2012, 87(1):218-226.
    [3] Chen X, Jiang HR, Yang YM, Liu N. Effect of exopolysaccharide from Bifidobacterium bifidum on cell of gastric cancer and human telomerase reverse transcriptase. Acta Microbiologica Sinica, 2009, 49(1):117-122. (in Chinese)陈旭, 江虹锐, 杨艳梅, 刘宁. 两歧双歧杆菌胞外多糖对胃癌细胞及端粒酶逆转录酶的影响. 微生物学报, 2009, 49(1):117-122.
    [4] Zheng YF, Zhang Q, Liu XM, Ma L, Lai F. Extraction of polysaccharides and its antitumor activity on Magnolia kwangsiensis Figlar & Noot. Carbohydrate Polymers, 2016, 142:98-104.
    [5] Liu J. Fermentation conditions, structures, chemical modification and antioxidant activity of exopolysaccharides from Paenibacillus polymyxa. Doctor Dissertation of Nanjing Agricultural University, 2010. (in Chinese)刘俊. 多粘类芽孢杆菌胞外多糖的发酵条件、结构、化学修饰及其抗氧化活性的研究. 南京农业大学博士学位论文, 2010.
    [6] Wang XJ, Wei CW, Xu SY, Lv MX, Zeng HP. Progress in polysaccharides chemical structure and structure-acivity. Guangzhou Chemical Industry, 2004, 32(1):6-10. (in Chinese)王晓娟, 魏传晚, 徐淑永, 吕梅香, 曾和平. 生物活性多糖结构与功效关系的研究进展. 广州化工, 2004, 32(1):6-10.
    [7] Guo LD. Advances of researches on endophytic fungi. Mycosystema, 2001, 20(1):148-152. (in Chinese)郭良栋. 内生真菌研究进展. 菌物系统, 2001, 20(1):148-152.
    [8] Zhang Y, Han T, Ming Q, Wu L, Rahman K, Qin L. Alkaloids produced by endophytic fungi:a review. Natural Product Communications, 2012, 7(7):963-968.
    [9] Mahapatra S, Banerjee D. Evaluation of in vitro antioxidant potency of exopolysaccharide from endophytic Fusarium solani SD5. International Journal of Biological Macromolecules, 2013, 53:62-66.
    [10] Pan BF, Su X, Hu B, Yang N, Chen Q, Wu W. Fusarium redolens 6WBY3, an endophytic fungus isolated from Fritillaria unibracteata var. wabuensis, produces peimisine and imperialine-3β-D-glucoside. Fitoterapia, 2015, 103:213-221.
    [11] Zha XQ, Xiao JJ, Zhang HN, Wang JH, Pan LH, Yang XF, Luo JP. Polysaccharides in Laminaria japonica (LP):extraction, physicochemical properties and their hypolipidemic activities in diet-induced mouse model of atherosclerosis. Food Chemistry, 2012, 134(1):244-252.
    [12] Hromádková Z, Ebringerová A, Valachovič P. Ultrasound-assisted extraction of water-soluble polysaccharides from the roots of valerian (Valeriana officinalis L.). Ultrasonics Sonochemistry, 2002, 9(1):37-44.
    [13] Liu L, Lu YM, Li XH, Zhou LY, Yang D, Wang LM, Chen Y. A novel process for isolation and purification of the bioactive polysaccharide TLH-3' from Tricholoma lobayense. Process Biochemistry, 2015, 50(7):1146-1151.
    [14] Lv Y, Yang XB, Zhao Y, Ruan, Y, Yang Y, Wang ZZ. Separation and quantification of component monosaccharides of the tea polysaccharides from Gynostemma pentaphyllum by HPLC with indirect UV detection. Food Chemistry, 2009, 112(3):742-746.
    [15] Li W, Ji J, Xu XY, Hu B, Wang K, Xu DL, Dong MS. Extraction and antioxidant activity in vitro of capsular polysaccharide from Lactobacillus helveticus MB2-1 in sayram yogurt from Xinjiang. Food Science, 2012, 33(21):34-38. (in Chinese)李伟, 纪鹃, 徐希研, 胡波, 王凯, 徐冬兰, 董明盛. 源自新疆赛里木酸奶的瑞士乳杆菌MB2-1荚膜多糖提取及其抗氧化活性. 食品科学, 2012, 33(21):34-38.
    [16] Chun SS, Vattem DA, Lin YT, Shetty K. Phenolic antioxidants from clonal oregano (Origanum vulgare) with antimicrobial activity against Helicobacter pylori. Process Biochemistry, 2005, 40(2):809-816.
    [17] Bayliak MM, Lylyk MP, Vytvytska OM, Lushchak VI. Assessment of antioxidant properties of alpha-keto acids in vitro and in vivo. European Food Research and Technology, 2016, 242(2):179-188.
    [18] Ge Y, Duan YF, Fang GZ, Zhang Y, Wang S. Polysaccharides from fruit calyx of Physalis alkekengi var. francheti:isolation, purification, structural features and antioxidant activities. Carbohydrate Polymers, 2009, 77(2):188-193.
    [19] Ghasemlou M, Khodaiyan F, Jahanbin K, Gharibzahedi SMT, Taheri S. Structural investigation and response surface optimisation for improvement of kefiran production yield from a low-cost culture medium. Food Chemistry, 2012, 133(2):383-389.
    [20] Sun WJ, Meng K, Qi CH, Yang XY, Wang YG, Fan WT, Yan ZG, Zhao XN. Liu JZ. Immune-enhancing activity of polysaccharides isolated from Atractylodis macrocephalae Koidz. Carbohydrate Polymers, 2015, 126:91-96.
    [21] Kačuráková M, Capek P, Sasinková V, Wellner, N, Ebringerová A. FT-IR study of plant cell wall model compounds:pectic polysaccharides and hemicelluloses. Carbohydrate Polymers, 2000, 43(2):195-203.
    [22] Zheng YF, Zhang S, Wang Q, Lu X, Lin LM, Tian YT, Xiao JB, Zheng BD. Characterization and hypoglycemic activity of a β-pyran polysaccharides from bamboo shoot (Leleba oldhami Nakal) shells. Carbohydrate Polymers, 2016, 144:438-446.
    [23] Xing XH, Cui SW, Nie SP, Phillips GO, Goff HD, Wang Q. Study on Dendrobium officinale O-acetyl-glucomannan (Dendronan®):Part I. Extraction, purification, and partial structural characterization. Bioactive Carbohydrates and Dietary Fibre, 2014, 4(1):74-83.
    [24] Strobel G, Stierle A, Stierle D, Hess WM. Taxomyces andreanae, a proposed new taxon for a bulbilliferous hyphomycete associated with Pacific yew (Taxus brevifolia). Mycotaxon, 1993, 47:71-80.
    [25] Chen Q. Studies on endophytic fungi and its secondary metabolites from bulbus Fritillariae Cirrhosae. Master dissertation of Sichuan Agricultural University, 2012. (in Chinese)陈鹊. 川贝母内生真菌及其次生代谢产物的研究. 四川农业大学硕士学位论文, 2012.
    [26] Pan F, Hou K, Gao F, Hu B, Chen Q, Wu W. Peimisine and peiminine production by endophytic fungus Fusarium sp. isolated from Fritillaria unibracteata var. wabensis. Phytomedicine, 2014, 21(8/9):1104-1109.
    [27] Chen Y, Mao WJ, Tao HW, Zhu WM, Qi XH, Chen YL, Li HY, Zhao CQ, Yang YP, Hou YJ, Wang CY, Li N. Structural characterization and antioxidant properties of an exopolysaccharide produced by the mangrove endophytic fungus Aspergillus sp. Y16. Bioresource Technology, 2011, 102(17):8179-8184.
    Cited by
    Comments
    Comments
    分享到微博
    Submit
Get Citation

Feng Pan, Yunxin Yao, Xin Tang, Wei Wu. Physicochemical property and antioxidant activity of exopolysaccharide produced by endophytic fungal Fusarium redolens 6WBY3 isolated from Fritillaria unibracteata var. wabuensis. [J]. Acta Microbiologica Sinica, 2017, 57(2): 240-253

Copy
Share
Article Metrics
  • Abstract:1227
  • PDF: 2092
  • HTML: 651
  • Cited by: 0
History
  • Received:June 16,2016
  • Revised:September 05,2016
  • Online: January 19,2017
Article QR Code
  • WeChat ID
  • Mobile Terminal

Acta Microbiologica Sinica ® 2025 All Rights Reserved