Chenyang Lu
School of Marine Sciences, Ningbo University, Ningbo 315211, Zhejiang Province, China;State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, ChinaHang Wu
School of Life Sciences, Anhui University, Hefei 230029, Anhui Province, ChinaXiurong Su
School of Marine Sciences, Ningbo University, Ningbo 315211, Zhejiang Province, ChinaLinquan Bai
State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, China[Objective] The effect of continuous low-expression region on validamycin A titer and biomass was evaluated through gene deletion experiments.[Methods] The deletion boundaries of large chromosome region were identified via transcriptome analysis. Meanwhile, deletion mutant was obtained via Cre-loxP system, titer of validamycin A was detected by HPLC and, biomass was represented by dry cell weight.[Results] The 1.2 Mb large region identified via transcriptome analysis was successfully deleted by Cre-loxP system. In comparison with parent strain TL01, deletion of 1.2 Mb region increased the biomass by 44% and showed no effect on validamycin A titer.[Conclusion] The strategy developed here can be applied to identify deletion boundaries of large chromosome region, and mutant of 1.2 Mb region deletion showed potential as host for heterologous expression of aminocyclitol biosynthetic gene clusters.
Chenyang Lu, Hang Wu, Xiurong Su, Linquan Bai. Effect of transcriptome-based large region deletion on validamycin A overproduction by Streptomyces hygroscopicus var. jinggangensis TL01. [J]. Acta Microbiologica Sinica, 2017, 57(7): 1038-1048
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