Effect of heavy metal Cu on Allogynogenetic Carassius auratus intestinal microorganism

doi:10.13343/j.cnki.wsxb.20160414

Home > Archive>Volume 57, Issue 7, 2017 >1060-1068. DOI:10.13343/j.cnki.wsxb.20160414

Effect of heavy metal Cu on Allogynogenetic Carassius auratus intestinal microorganism
DOI:
                        10.13343/j.cnki.wsxb.20160414
                    
CSTR:
                        32112.14.j.AMS.20160414
                    
Author:
                        Zongcai TuZongcai Tu
Key Laboratory of Functional Small Organic Molecule, Ministry of Education, College of Life Sciences, Jiangxi Normal University, Nanchang 330022, Jiangxi Province, China;State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, Jiangxi Province, China
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Juanjuan PangJuanjuan Pang
Key Laboratory of Functional Small Organic Molecule, Ministry of Education, College of Life Sciences, Jiangxi Normal University, Nanchang 330022, Jiangxi Province, China
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Hui WangHui Wang
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, Jiangxi Province, China
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Lu ZhangLu Zhang
Key Laboratory of Functional Small Organic Molecule, Ministry of Education, College of Life Sciences, Jiangxi Normal University, Nanchang 330022, Jiangxi Province, China
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Xiaomei ShaXiaomei Sha
Key Laboratory of Functional Small Organic Molecule, Ministry of Education, College of Life Sciences, Jiangxi Normal University, Nanchang 330022, Jiangxi Province, China
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Abstract:

[Objective] To study the effect of the heavy metal Cu on the bacterial composition and diversity in Allogynogenetic Carassius auratus intestinal.[Methods] Total DNA was extracted by DNA extraction kit. The 16S rRNA gene of intestinal microbial diversity was amplified by universal PCR primers. Then the clone library was built to analyze data.[Results] The main bacterial community in Allogynogenetic Carassius auratus intestinal contained Firmicutes, Proteobacteria and Bacteroidetes. At different concentrations of Cu, the contents of Firmicutes in Allogynogenetic Carassius auratus gut significantly decreased. The results of Rarefaction Curyiyuyiyuve, Venn and Diversity index showed that gut microbial diversity decreased obviously.[Conclusion] Cu caused the microbial diversity in Allogynogenetic Carassius auratus decreased. This result provides a basis for further studies on the health of Allogynogenetic Carassius auratus relevant to heavy metal Cu.

Key words:Allogynogenetic Carassius auratus;intestinal microflora;heavy metal copper

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Zongcai Tu, Juanjuan Pang, Hui Wang, Lu Zhang, Xiaomei Sha. Effect of heavy metal Cu on Allogynogenetic Carassius auratus intestinal microorganism. [J]. Acta Microbiologica Sinica, 2017, 57(7): 1060-1068

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Received:October 15,2016
Revised:February 14,2017
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Online: July 07,2017
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