Comparative proteomics reveals the role of NDM-1 in Acinetobacter baumannii

Abstract:

[Objective] In order to investigate how NDM-1 affect the metabolism of A. baumannii, we analyzed the differentially expressed proteins (DEPs) between clinically isolated multidrug-resistant A. baumannii MDR-ZJ06 (bla_NDM-1^-) and ABC3229 (bla_NDM-1⁺) which have a close genetic relationship. [Methods] We used 2-DE coupled with MALDI-TOF MS/MS to analyze the differentially expressed proteins (DEPs) between A. baumannii MDR-ZJ06 and ABC3229. Based on GO annotations, we analyze DEPs by KEGG, Function classification, enrichment analysis and protein-protein interaction network. [Results] We found that there were 51 DEPs, including 11 up-regulated proteins and 41 down-regulated proteins in ABC3229. These proteins were predominantly involved in reducing carbohydrate metabolism, amino acid metabolism, fatty acid metabolism, cell wall synthesis, and increasing of iron transport system formed. [Conclusion] These results uncover the effect of NDM-1 on bacteria may be resistant to antibiotics by slowing down the metabolism of bacteria and increasing the uptake of iron, thereby the bacteria will systemic resistance antibiotics.

Key words:Acinetobacter baumannii;NDM-1;multidrug resistance;comparative proteomics