Genomic analysis reveals the biosynthesis pathways of diverse secondary metabolites and pinoresinol and its glycoside derivatives in Phomopsis sp.XP-8

Abstract:

[Objective] Sequencing and analysis of Phomopsis sp. XP-8 genome are beneficial to reveal the potential metabolic pathways of this strain and the key genes related to the biosynthesis of pinoresinol and its glycoside derivatives and other secondary metabolites. [Methods] We sequenced Phomopsis sp. XP-8 genome by the Illumina HiSeq 2500 high throughput sequencing platform. Then gene prediction and functional annotation were analyzed using different softwares. [Results] The final assembled genome size was approximately 55.2 Mb with an overall GC content of 53.5%. Further annotation analyses predicted 17094 protein-coding genes and 310 non-coding RNA genes. A large set of candidate genes involved in the production of pinoresinol, its glycoside derivatives and other secondary metabolites were identified. Orthology and phylogenetic analysis revealed that Phomopsis sp. XP-8 and 5 Ascomycota share 12635 orthologous genes and 5626 gene families. [Conclusion] Phomopsis sp. XP-8 possessed genomic basis for production of diverse secondary metabolites, including pinoresinol and its glycoside derivatives. This study provides basis for the further metabolic engineering of pinoresinol and its glucoside production.

Key words:Phomopsis sp.;endophytic fungi;secondary metabolites;lignan;Illumina sequencing