Physiological and transcriptional responses of Actinobacillus succinogenes to acid stress
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    Abstract:

    [Objective] We explored by physiological and transcriptional responses of Actinobacillus succinogenes CGMCC1593 to acid stress. [Methods] We studied the effects of different initial pH on cell growth, activity of H+-ATPase and intracellular pH before and after acid stress as well as the protection mechanism of glutamate on CGMCC1593. The transcriptomics technique was used to explore the differentially expressed genes under acid stress. [Results] Cell growth was inhibited and the activity of H+-ATPase decreased with the decrease of initial pH. After acid stress at pH 4.7, the cell membrane was severely damaged. Glutamate had a protective effect on the cells under acid stress. In total 39 genes were differently expressed altogether under acid stress, of which 49% were stress and transport proteins, and a small part was related to metabolism. [Conclusion] Understanding the physiological and transcriptional responses of Actinobacillus succinogenes to acid stress provides references for improving acid resistance of Actinobacillus succinogenes.

    Reference
    [1] Cok B, Tsiropoulos I, Roes AL, Patel MK. Succinic acid production derived from carbohydrates:an energy and greenhouse gas assessment of a platform chemical toward a bio-based economy. Biofuels Bioproducts & Biorefining, 2014, 8(1):16-29.
    [2] Choi S, Song CW, Shin JH, Lee SY. Biorefineries for the production of top building block chemicals and their derivatives. Metabolic Engineering, 2015, 28:223-239.
    [3] Guettler MV, Rumler D, Jain MK. Actinobacillus succinogenes sp. nov., a novel succinic-acid-producing strain from the bovine rumen. International Journal of Systematic and Evolutionary Microbiology, 1999, 49:207-216.
    [4] Liu YP, Zheng P, Sun ZH, Ni Y, Dong JJ, Wei P. Strategies of pH control and glucose-fed batch fermentation for production of succinic acid by Actinobacillus succinogenes CGMCC1593. Journal of Chemical Technology & Biotechnology, 2008, 83(5):722-729.
    [5] Liu X, Zheng P, Ni Y, Dong JJ, Sun ZH. Breeding Actinobacillus succinogenes with acid-tolerance by genome shuffling. Microbiology, 2009, 36(11):1676-1681. (in Chinese) 刘璇, 郑璞, 倪晔, 董晋军, 孙志浩. 基因组改组技术选育耐酸性琥珀酸放线杆菌. 微生物学通报, 2009, 36(11):1676-1681.
    [6] Breeuwer P, Drocourt J, Rombouts FM, Abee T. A novel method for continuous determination of the intracellular pH in bacteria with the internally conjugated fluorescent probe 5(and 6-)-carboxyfluorescein succinimidyl ester. Applied & Environmental Microbiology, 1996, 62(1):178-183.
    [7] Belli WA, Marquis RE. Adaptation of Streptococcus mutans and Enterococcus hirae to acid stress in continuous culture. Applied and Environmental Microbiology, 1991, 57(4):1134-1138.
    [8] Hersh BM, Farooq FT, Barstad DN, Blankenhorn DL, Slonczewski JL. A glutamate-dependent acid resistance gene in Escherichia coli. Journal of Bacteriology, 1996, 178(13):3978-3981.
    [9] Wu CD, Zhang J, Wang M, Du GC, Chen J. Lactobacillus casei combats acid stress by maintaining cell membrane functionality. Journal of Industrial Microbiology & Biotechnology, 2012, 39(7):1031-1039.
    [10] Castanie-Cornet MP, Penfound TA, Smith D, Elliott JF, Foster JW. Control of acid resistance in Escherichia coli. Journal of Bacteriology, 1999, 181(11):3525-3535.
    [11] Martín-Galiano AJ, Ferrándiz MJ, de la Campa AG. The promoter of the operon encoding the F0F1 ATPase of Streptococcus pneumoniae is inducible by pH. Molecular Microbiology, 2001, 41(6):1327-1338.
    [12] Cusumano ZT, Caparon MG. Citrulline protects Streptococcus pyogenes from acid stress using the arginine deiminase pathway and the F1F0-ATPase. Journal of Bacteriology, 2015, 197(7):1288-1296.
    [13] Wilson CM, Loach D, Lawley B, Bell T, Sims IM, O'Toole PW, Zomer A, Tannock GW. Lactobacillus reuteri 100-23 modulates urea hydrolysis in the murine stomach. Applied and Environmental Microbiology, 2014, 80(19):6104-6113.
    [14] de Lucena DKC, Pühler A, Weidner S. The role of sigma factor RpoH1 in the pH stress response of Sinorhizobium meliloti. BMC Microbiology, 2010, 10(1):265.
    [15] Abdullah-Al-Mahin, Sugimoto S, Higashi C, Matsumoto S, Sonomoto K. Improvement of multiple-stress tolerance and lactic acid production in Lactococcus lactis NZ9000 under conditions of thermal stress by heterologous expression of Escherichia coli DnaK. Applied and Environmental Microbiology, 2010, 76(13):4277-4285.
    [16] Harrison CJ, Hayer-Hartl M, Di Liberto M, Hartl FU, Kuriyan J. Crystal structure of the nucleotide exchange factor GrpE bound to the ATPase domain of the molecular chaperone DnaK. Science, 1997, 276(5311):431-435.
    [17] Brehmer D, Rüdiger S, Gässler CS, Klostermeier D, Packschies L, Reinstein J, Mayer MP, Bukau B. Tuning of chaperone activity of Hsp70 proteins by modulation of nucleotide exchange. Nature Structural Biology, 2001, 8(5):427-432.
    [18] Zolkiewski M. A camel passes through the eye of a needle:protein unfolding activity of Clp ATPases. Molecular Microbiology, 2006, 61(5):1094-1100.
    [19] Meibom KL, Dubail I, Dupuis M, Barel M, Lenco J, Stulik J, Golovliov I, Sjöstedt A, Charbit A. The heat-shock protein ClpB of Francisella tularensis is involved in stress tolerance and is required for multiplication in target organs of infected mice. Molecular Microbiology, 2008, 67(6):1384-1401.
    [20] Suo YK, Luo S, Zhang YN, Liao ZP, Wang JF. Enhanced butyric acid tolerance and production by Class I heat shock protein-overproducing Clostridium tyrobutyricum ATCC 25755. Journal of Industrial Microbiology & Biotechnology, 2017, 44(8):1145-1156.
    [21] Choi SH, Baumler DJ, Kaspar CW. Contribution of dps to acid stress tolerance and oxidative stress tolerance in Escherichia coli O157:H7. Applied & Environmental Microbiology, 2000, 66(9):3911-3916.
    [22] Clarke MB, Sperandio V. Transcriptional autoregulation by quorum sensing Escherichia coli regulators B and C (QseBC) in enterohaemorrhagic E. coli (EHEC). Molecular Microbiology, 2005, 58(2):441-455.
    [23] Yin X, Feng Y, Lu Y, Chambers JR, Gong J, Gyles CL. Adherence and associated virulence gene expression in acid-treated Escherichia coli O157:H7in vitro and in ligated pig intestine. Microbiology, 2012, 158(Pt 4):1084-1093.
    [24] Leaphart AB, Thompson DK, Huang K, Alm E, Wan XF, Arkin A, Brown SD, Wu LY, Yan TF, Liu XD, Wickham GS, Zhou JZ. Transcriptome profiling of Shewanella oneidensis gene expression following exposure to acidic and alkaline pH. Journal of Bacteriology, 2006, 188(4):1633-1642.
    [25] Carpenter CE, Broadbent JR. External concentration of organic acid anions and pH:key independent variables for studying how organic acids inhibit growth of bacteria in mildly acidic foods. Journal of Food Science, 2009, 74(1):R12-R15.
    [26] Chen J, Zhu XN, Tan ZG, Xu HT, Tang JL, Xiao DG, Zhang XL. Activating C4-dicarboxylate transporters DcuB and DcuC for improving succinate production. Applied Microbiology & Biotechnology, 2014, 98(5):2197-2205.
    [27] Nie RX, Stark S, Symersky J, Kaplan RS, Lu M. Structure and function of the divalent anion/Na+ symporter from Vibrio cholerae and a humanized variant. Nature Communications, 2017, 8:15009.
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Qun Zhang, Pengcheng Chen, Pu Zheng. Physiological and transcriptional responses of Actinobacillus succinogenes to acid stress. [J]. Acta Microbiologica Sinica, 2018, 58(7): 1255-1265

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History
  • Received:August 15,2017
  • Revised:October 18,2017
  • Online: July 05,2018
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