Integrated analysis of nitrite-dependent anaerobic methane oxidation bacteria

Abstract:

[Objective] In order to give a full overview including ecological distribution, community structure and phylogenic diversity.[Methods] All n-damo bacteria related sequences from NCBI databases (data collected until November 2016) and together with our experimental data from Dajiuhu surface peat samples through 16S rRNA illuminia sequencing were set to do the bioinformatics analysis.[Results]n-damo bacteria were mainly detected in the sediment, wetlands and paddy soil. The average detection rate of n-damo bacteria based on pmoA gene was 7 times more than that based on 16S rRNA gene, whereas Shannon-Wiener index (1.4-3.4) remained relatively stable. The abundance of NC10 was only 0.067% from Dajiuhu peatland, which was the lowest abundance in all published data, indicating nitrogen was a limiting factor for NC10. Group A including Methylomirabilis oxyfera which had been test to have the anaerobic methane oxidation accounts for only a little bit (less than 20%), suggesting that the real ecological potential of methane consumption by n-damo bacteria need more deep study. [Conclusion] The integrated analysis on n-damo bacteria deepen our knowledge of this functional group and may give some guideline for further study.

Key words:nitrite-dependent anaerobic methane oxidation bacteria (n-damo bacteria);integrated analysis;community structure;phylogenic analysis