Xuri Dong
College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaXufeng Chai
College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaLing Tan
College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaJiawei Qi
College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaHaiquan Liu
College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaYingjie Pan
College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China;Safety Risk Assessment Laboratory of Aquatic-Product Processing & Preservation, Ministry of Agriculture, Shanghai 201306, China;Engineering Research Center of Food Thermal-Processing Technology, Shanghai Ocean University, Shanghai 201306, ChinaYong Zhao
College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China;Safety Risk Assessment Laboratory of Aquatic-Product Processing & Preservation, Ministry of Agriculture, Shanghai 201306, China;Engineering Research Center of Food Thermal-Processing Technology, Shanghai Ocean University, Shanghai 201306, China[Objective] We studied the formation of a mixed biofilm formed by Vibrio parahaemolyticus and Vibrio cholerae. [Methods] Biofilm formation was quantified using crystal violet staining, abundance of extracellular polysaccharide and extracellular protein, fluorescence in situ hybridization and colony plate count. Biofilms formed by individual and mixed strains were determined from the start and the following intervals at 4, 8, 12, 24, 36, 48, 60 and 72 h. [Results] The amount of biofilm produced by the mixed strains was less than the total amount of biofilm produced by the individual strains, indicating there was no synergism between the strains. However, in the mixed biofilm the time of maturation was increased, the stability of the biofilm to external perturbation was enhanced and the cell density was higher. [Conclusion] The biofilm formed by a mixed population of V. parahaemolyticus and V. cholera is therefore more persistent and can potentially pose a greater risk for recontamination and subsequent development of disease.
Xuri Dong, Xufeng Chai, Ling Tan, Jiawei Qi, Haiquan Liu, Yingjie Pan, Yong Zhao. Dynamic biofilm forming process of Vibrio parahaemolyticus and Vibrio cholera. [J]. Acta Microbiologica Sinica, 2018, 58(10): 1808-1816
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