Diversity and community structure of ammonia oxidizing archaea in rhizosphere soil of reed in Ebinur Lake Wetland

Abstract:

[Objective] The aim of this study was to reveal the differences in the community structure of ammonia-oxidizing archaea (AOA) between rhizosphere and non-rhizosphere soil, to provide a theoretical basis for further study on the relationship between halophyte rhizosphere soil microorganisms and salt tolerance. [Methods] Using ammonia monooxygenase gene (amoA) as molecular marker, the diversity and community structure of AOA in rhizosphere soil of Reed in different seasons (spring, summer, autumn) in Ebinur Lake Wetland were studied by high-throughput sequencing technique. [Results] The diversity and richness of AOA were different in rhizosphere soil of Reed in different seasons. In summer and autumn, the richness of AOA in rhizosphere soil of Reed was higher than that in non-rhizosphere soil, the diversity was lower than that in non-rhizosphere soil. In spring, the diversity of AOA in rhizosphere soil of Reed was higher than that in non-rhizosphere soil, the richness was lower than that in non-rhizosphere soil. The diversity magnitude of AOA in terms of the season was spring > summer > autumn in rhizosphere soil of Reed. Community composition analysis of AOA showed that the main AOA communities in all soil samples were Crenarchaeota and Thaumarchaeota, and Crenarchaeota were the dominant phylum. RDA analysis showed that soil moisture, soil organic matter, total potassium and pH were the main factors affecting the diversity and richness of AOA community in rhizosphere soil of Reed.[Conclusion] The diversity and richness of AOA in rhizosphere soil of Reed were season dependent, compared with non-rhizosphere soil, AOA was more abundant in rhizosphere soil of Reed.

Key words:Ebinur Lake Wetland;rhizosphere and non-rhizosphere soil;ammonia-oxidizing archaea (AOA);diversity and richness;correlation analysis