Characterization of Serine/Threonine protein kinases K (PknK) in Mycobacterium

doi:10.13343/j.cnki.wsxb.20190051

Home > Archive>Volume 59, Issue 12, 2019 >2378-2389. DOI:10.13343/j.cnki.wsxb.20190051

Characterization of Serine/Threonine protein kinases K (PknK) in Mycobacterium
DOI:
                        10.13343/j.cnki.wsxb.20190051
                    
CSTR:
                        32112.14.j.AMS.20190051
                    
Author:
                        Yixuan ZhouYixuan Zhou
CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China;Savaid Medical School, University of Chinese Academy of Sciences, Beijing 101408, China
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Xintong ZhouXintong Zhou
CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
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Jian YangJian Yang
CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
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Xinling HuXinling Hu
CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
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Kaixia MiKaixia Mi
CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China;Savaid Medical School, University of Chinese Academy of Sciences, Beijing 101408, China
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Abstract:

[Objective] Serine/Threonine Protein Kinases K (PknK) is a eukaryotic-like Ser/Thr protein kinase in Mycobacterium, with important roles in cell growth and signaling transduction. However, its underlying mechanism of action is not completed. [Methods] The pknK knockout strain △pknK was obtained by phage specialized transduction meanwhile the complement strain pMV361-pknK/ΔpknK and the overexpressing strain with pMV261-pknK/BCG was construed for further analysis. The growth curve and resistance of the obtained strains were determined. PknK-interacting proteins were identified by pulldown-MS. [Results] PknK affected Mycobacterium growth, and △pknK had growth advantage over BCG and pMV261-pknK/BCG. Knockout pknK led to increased multiple antibiotic susceptibility. We identified the binding proteins for PnK in BCG using in pulldown combined with Mass Spectrometry. [Conclusion] PknK negatively regulates BCG growth and increases antibiotic susceptibility in mycobacteria. The PknK binding proteins include a Ser/Thr protein kinase PknA, and two component system regulators such as MtrA, MoxR1 and TrcR, which is expected to be a resource for understanding the PknK-mediated signaling pathways in Mycobacterium, thus facilitating new therapeutic strategies for antibiotic-resistant infections.

Key words:STPKs;PknK;growth;antibiotic resistance

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Yixuan Zhou, Xintong Zhou, Jian Yang, Xinling Hu, Kaixia Mi. Characterization of Serine/Threonine protein kinases K (PknK) in Mycobacterium. [J]. Acta Microbiologica Sinica, 2019, 59(12): 2378-2389

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Received:January 30,2019
Revised:March 25,2019
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Online: December 03,2019
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