Full genome sequence of Chinese date mosaic-associated virus, a new member of the genus Emaravirus
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    Abstract:

    [Objective] A new jujube disease has emerged in Aksu and other areas in Xinjiang since 2016, seriously threatening the local and surrounding jujube industries during the last three years. This study aims to identify the causative pathogen(s) and to investigate the pathogen transmission route(s), therefore providing guides for the disease control strategies. [Methods] Small RNA sequencing was performed to identify potential pathogen(s), which revealed a new jujube virus. RNAseq and reverse transcription PCR (RT-PCR) were performed to obtain the whole genomic sequence of the identified virus. A viral protein was in-vitro expressed for antibody preparation. Western blotting with the viral specific antibody confirmed the specific existence of the viral protein in infected plants. Insects were collected in the disease-occurred areas and RT-PCR was performed to identify potential virus-transmission vector(s).[Results] This study identified a new virus, belonging to Emaravirus and named as Chinese date mosaic-associated virus (CDMaV) in this study, as a correlated causative regent for Xinjiang jujube disease. We obtained the whole genomic sequence of this viral strain. The CDMaV genome is segmented and consists of five segments of linear negative-sense and single-stranded RNA. The complete genome is 13.078 kb, with RNA1-RNA5 containing 7160 nt, 2224 nt, 1230 nt, 1493 nt, and 971 nt, respectively. The complementary strand of each genomic RNA encodes a single open reading frame, sequentially the five encoded proteins are RNA-dependent RNA polymerase, envelope glycoprotein, nucleocapsid protein, and two unknown functional proteins. The virus specific sequences could be amplified from Epitrimerus zizyphagus, indicated that CDMaV might be transmitted from infected jujube trees to the healthy plants via this insect vector. [Conclusion] This study identified CDMaV as the correlated causative agent for the newly emerged jujube disease in Xinjiang, sequenced the viral genome and identified E. zizyphagus as a potential insect vector for viral transmission. Identification of pathogen and its transmission route would efficiently guide the development of disease control strategies.

    Reference
    [1] Mielke N, Muehlbach HP. A novel, multipartite, negative-strand RNA virus is associated with the ringspot disease of European mountain ash (Sorbus aucuparia L.). The Journal of General Virology, 2007, 88(4):1337-1346.
    [2] Mielke-Ehret N, Mühlbach HP. Emaravirus:a novel genus of multipartite, negative strand RNA plant viruses. Viruses, 2012, 4(9):1515-1536.
    [3] Elbeaino T, Digiaro M, Martelli GP. Complete nucleotide sequence of four RNA segments of fig mosaic virus. Archives of Virology, 2009, 154(11):1719-1727.
    [4] Elbeaino T, Digiaro M, Uppala M, Sudini H. Deep sequencing of dsRNAs recovered from mosaic-diseased pigeonpea reveals the presence of a novel emaravirus:pigeonpea sterility mosaic virus 2. Archives of Virology, 2015, 160(8):2019-2029.
    [5] Kumar PL, Jones AT, Reddy DVR. A novel mite-transmitted virus with a divided RNA genome closely associated with pigeonpea sterility mosaic disease. Phytopathology, 2003, 93(1):71-81.
    [6] McGavin WJ, Mitchell C, Cock PJA, Wright KM, MacFarlane SA. Raspberry leaf blotch virus, a putative new member of the genus Emaravirus, encodes a novel genomic RNA. The Journal of General Virology, 2012, 93(2):430-437.
    [7] Zerbino DR, Birney E. Velvet:algorithms for de novo short read assembly using de Bruijn graphs. Genome Research, 2008, 18(5):821-829.
    [8] Zhang ZX, Qi SS, Tang N, Zhang XX, Chen SS, Zhu PF, Ma L, Cheng JP, Xu Y, Lu MG, Wang HQ, Ding SW, Li SF, Wu QF. Discovery of replicating circular RNAs by RNA-seq and computational algorithms. PLoS Pathogens, 2014, 10(12):e1004553.
    [9] Barba M, Czosnek H, Hadidi A. Historical perspective, development and applications of next-generation sequencing in plant virology. Viruses, 2014, 6(1):106-136.
    [10] Roossinck MJ, Martin DP, Roumagnac P. Plant virus metagenomics:advances in virus discovery. Phytopathology, 2015, 105(6):716-727.
    [11] Lu YW, McGavin W, Cock PJA, Schnettler E, Yan F, Chen JP, MacFarlane S. Newly identified RNAs of raspberry leaf blotch virus encoding a related group of proteins. The Journal of General Virology, 2015, 96(11):3432-3439.
    [12] Zheng YZ, Navarro B, Wang GP, Wang YX, Yang ZK, Xu WX, Zhu CX, Wang LP, Di Serio F, Hong N. Actinidia chlorotic ringspot-associated virus:a novel emaravirus infecting kiwifruit plants. Molecular Plant Pathology, 2017, 18(4):569-581.
    [13] Tatineni S, McMechan AJ, Wosula EN, Wegulo SN, Graybosch RA, French R, Hein GL. An eriophyid mite-transmitted plant virus contains eight genomic RNA segments with unusual heterogeneity in the nucleocapsid protein. Journal of Virology, 2014, 88(20):11834-11845.
    [14] Di Bello PL, Laney AG, Druciarek T, Ho T, Gergerich RC, Keller KE, Martin RR, Tzanetakis IE. A novel emaravirus is associated with redbud yellow ringspot disease. Virus Research, 2016, 222:41-47.
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Da Liu, Jianyu Bai, Rongxiang Fang, Lili Zhang. Full genome sequence of Chinese date mosaic-associated virus, a new member of the genus Emaravirus. [J]. Acta Microbiologica Sinica, 2020, 60(2): 397-405

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History
  • Received:April 22,2019
  • Revised:June 14,2019
  • Online: February 13,2020
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