Targeted exploration of Streptomyces lavendulae subsp. CQ01819 and its secondary metabolites

Abstract:

[Objective] To obtain targeted natural products and the bacterial producers based on characteristic biosynthesis genes and transcription level analysis. [Methods] First, we directionally mined the secondary metabolites producers by 5 pair of degenerate primers according to the conserved biosynthesis genes. Then RT-qPCR guided fermentation medium optimization was performed. Finally, the targeted natural products were isolated using different chromatography technologies, and their chemical structures were identified based on High Resolution Mass Spectrometry (HRMS) and Nuclear Magnetic Resonance (NMR).[Results] The AHBA synthase and epoxidase-encoding genes positive Streptomyces lavendulae subsp. CQ01819 was successfully obtained from soil. Targeted compounds purification led to discovering AHBA-derived mitomycin C, polyether antibiotic monensin A and valindolmycin.[Conclusion] Our findings provide a good example for efficiently discovering bacterial resource of expected natural products.

Key words:directional strains isolation;Streptomyces;gene transcription analysis;medium optimization;structure elucidation