Physicochemical properties of rabbit hemorrhagic disease virus type 2

Abstract:

[Objective] The purpose of this study was to study the physicochemical properties of the virus RHDV2 and evaluate effect of different treatment to kill the virus. [Methods] The virus was identified by RT-PCR in rabbits with clinical suspected death from RHDV2 infection. The physicochemical properties of RHDV2 were studied by PMA-RT-qPCR at different pH, different temperatures, common veterinary disinfectants and different concentrations of formaldehyde. [Results] RHDV2 infection was confirmed by RT-PCR detection and sequencing analysis. The VP60 gene sequence of this strain was 98.35% consistent with RHDV2 in GenBank (MN276176.1). PMA-RT-qPCR results show that RHDV2 was sensitive to pH, high temperature, animal disinfectant and formaldehyde. The killing rate of virus increased with pH decreasing or increasing. The virus killing rate reached 77.61% after being treated at 65℃ for 30 min, and 95.10% after being treated at 83℃ for 5 min. RHDV2 is sensitive to all three animal disinfectants, among which povidone iodine had the best killing effect on RHDV2, the virus killing rate reached 88.78% after 30 min. The killing rate of 0.3% formaldehyde was better than 0.2%, and after 30 min of application, the killing rate could reach 82.22%. [Conclusion] RHDV2 is sensitive to acid and base, high temperature, animal disinfectant and formaldehyde to varying degrees.

Key words:rabbit hemorrhagic disease virus 2;physical and chemical characteristics;PMA-RT-qPCR;disinfectant