Home > Archive>Volume 61, Issue 7, 2021 >2101-2111. DOI:10.13343/j.cnki.wsxb.20200606
PDF HTML XML Export Cite reminder
Phenotypic and genetic diversity of Photobacterium damsela subsp. damsela isolated from aquatic animals in Hainan Province and Bohai Sea region
Author:
  • Article
    • | |
  • Metrics
    • |
  • Reference [28]
    • |
  • Related
    • | | |
  • Comments
    Abstract:

    Photobacterium damselae subsp. damselae (PDD) is an important pathogen distributed widely in global marine environment, which could infect multiple marine organisms, and reported in various mariculture regions of China in recent years. [Objective] This study aimed to systematically compare the phenotypic and genetic diversity of PDD isolated from Hainan province and Bohai Sea region base on physiological and metabolic phenotype, virulence gene distribute and molecular genetic analysis.[Methods] The physiological and metabolic phenotypes of sucrose fermentation, moveability, hemolysis, and phospholipase activity were assayed. The profiles of pPHDD1 replication origin, dly, hlyApl, hlyAch and plpV were investigated by PCR detection. Genetic diversity of PDD was analyzed based on the toxR sequences. [Results] Four PDD strains were positive for sucrose fermentation while other twelve strains were negative. There are significant differences among the motility of PDD, in which strain Pdd1608, Pdd2009, and Pdd1704 showed strong motility. Pdd1608 isolated from Bohai Sea region exhibited strong hemolysis and phospholipase activities, whlie strain Pdd1612 and Pdd0912 isolated from Hainan Province showed weak hemolysis and none phospholipase activities, and 13 strains showed weak hemolysis and phospholipase activities. The virulence gene profile of Pdd1608 was pPHDD1 replication origin-dly-hlyApl-hlyAch-plpV, while other strains were hlyAch-plpV. Phylogenetic analysis suggested remarkable genetic diversity among different kinds of PDD. [Conclusion] PDD isolated from Hainan Province and Bohai Sea region were probably composed of a polyclonal bacterial population with complex phenotypic characterizations, similar virulence gene profiles, and high genetic diversity.

    Reference
    [1] Takahashi H, Miya S, Kimura B, Yamane K, Arakawa Y, Fujii T. Difference of genotypic and phenotypic characteristics and pathogenicity potential of Photobacterium damselae subsp. damselae between clinical and environmental isolates from Japan. Microbial Pathogenesis, 2008, 45(2):150-158.
    [2] Rivas AJ, Lemos ML, Osorio CR. Photobacterium damselae subsp. damselae, a bacterium pathogenic for marine animals and humans. Frontiers in Microbiology, 2013:283-283.
    [3] Love M, Teebken-Fisher D, Hose JE, Farmer JJ, Hickman FW, Fanning GR. Vibrio damsela, a marine bacterium, causes skin ulcers on the damselfish Chromis punctipinnis. Science, 1981, 214(4525):1139-1140.
    [4] Smith SK, Sutton DC, Fuerst JA, Reichelt JL. Evaluation of the genus Listonella and reassignment of Listonella damsela (Love et al.) MacDonell and Colwell to the genus Photobacterium as Photobacterium damsela comb. nov. with an emended description. International Journal of Systematic Bacteriology, 1991, 41(4):529-534.
    [5] Abdel-Aziz M, Eissa AE, Hanna M, Okada MA. Identifying some pathogenic Vibrio/Photobacterium species during mass mortalities of cultured Gilthead seabream (Sparus aurata) and European seabass (Dicentrarchus labrax) from some Egyptian coastal provinces. International Journal of Veterinary Science and Medicine, 2013, 1(2):87-95.
    [6] Sadok K, Faouzi L, Amina B, Kamel G. Virulence properties, biofilm formation and random amplified polymorphic DNA analysis of Photobacterium damselae subsp. damselae isolates from cultured sea bream (Sparus aurata) and seabass (Dicentrarchus labrax). Microbial Pathogenesis, 2014, 69/70:13-19.
    [7] Sharma SR, Pradeep MA, Sadu N, Dube PN, Vijayan KK. First report of isolation and characterization of Photobacterium damselae subsp. damselae from cage-farmed cobia (Rachycentron canadum). Journal of Fish Diseases, 2017, 40(7):953-958.
    [8] Chiu TH, Kao LY, Chen ML. Antibiotic resistance and molecular typing of Photobacterium damselae subsp. damselae, isolated from seafood. Journal of Applied Microbiology, 2013, 114(4):1184-1192.
    [9] Pedersen K, Skall HF, Lassennielsen AM, Bjerrum L, Olesen NJ. Photobacterium damselae subsp. damselae, an emerging pathogen in Danish rainbow trout, Oncorhynchus mykiss (Walbaum), mariculture. Journal of Fish Diseases, 2010, 32(5):465-472.
    [10] Terceti MS, Vences A, Matanza XM, Dalsgaard I, Pedersen K, OsorioCR. Molecular epidemiology of Photobacterium damselae subsp. damselae outbreaks in marine rainbow trout farms reveals extensive horizontal gene transfer and high genetic diversity. Frontiers in Microbiology, 2018.
    [11] Terceti MS, Ogut H, Osorio CR. Photobacterium damselae subsp. damselae, an emerging fish pathogen in the Black Sea:evidence of a multiclonal origin. Applied and Environmental Microbiology, 2016, 82(13):3736-3745.
    [12] Rivas AJ, Balado M, Lemos ML, Osorio CR. The Photobacterium damselae subsp. damselae hemolysins damselysin and HlyA are encoded within a new virulence plasmid. Infection and Immunity, 2011, 79(11):4617-4627.
    [13] Vences A, Rivas AJ, Lemos ML, Husmann M, Osorio CR. Chromosome-encoded hemolysin, phospholipase, and collagenase in plasmidless isolates of Photobacterium damselae subsp. damselae contribute to virulence for fish. Applied and Environmental Microbiology, 2017, 83(11).
    [14] 许悦. 海南地区养殖石斑鱼两种细菌性疾病病原病理学研究. 海南大学硕士学位论文, 2018.
    [15] Wang Z, Shi C, Wang H, Wan, XY, Zhang QL, Song XL, Li G, Gong M, Ye SG, Xie GS. A novel research on isolation and characterization of Photobacterium damselae subsp. damselae from Pacific white shrimp, Penaeus vannamei, displaying black gill disease cultured in China. Journal of Fish Diseases, 2020, 43(5):551-559.
    [16] 王凯. 长岛地区深水网箱养殖许氏平鲉主要疾病的流行病学调查与病原病理学研究. 上海海洋大学硕士学位论文, 2018.
    [17] Osorio CR, Toranzo AE, Romalde JL, Barja JL. Multiplex PCR assay for ureC and 16S rRNA genes clearly discriminates between both subspecies of Photobacterium damselae. Diseases of Aquatic Organisms, 2000, 40(3):177-183.
    [18] Osorio CR, Klose KE. A region of the transmembrane regulatory protein ToxR that tethers the transcriptional activation domain to the cytoplasmic membrane displays wide divergence among Vibrio species. Journal of Bacteriology, 2000, 182(2):526-528.
    [19] Kumar S, Stecher G, Tamura K. MEGA7:molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 2016, 33(7):1870-1874.
    [20] Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG. Clustal W and Clustal X version 2.0. Bioinformatics:Oxford, England, 2007, 23(21):2947-2948.
    [21] Saitou N, Nei M. The neighbor-joining method:a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 1987, 4(4):406-425.
    [22] Tamura K, Nei M, Kumar S. Prospects for inferring very large phylogenies by using the neighbor-joining method. Proceedings of the National Academy of Sciences of the United States of America, 2004, 101(30):11030-11035.
    [23] Felsenstein J. Confidence limits on phylogenies:an approach using the bootstrap. Evolution; International Journal of Organic Evolution, 1985, 39(4):783-791.
    [24] La?楥捬牬潡戠楁漬氠潂来楲换慥?匠楃測椠捍慡??楨???㈠き水????ぴ??ㄠ??㈠?は????????戮爠?孩??嵨??慯湢污潣湴?割呩???潤牡獭祳瑥桬敡??圯?￣?潳潵灢敳牰????￣?楡湭畳穥穬潡??刯?￣?攠污汮礠???呲???慧琠慰污?灨敯湧敥瑮爠慡瑦楦湥杣?獩歮楧渠?略汷挠散牵獬?極湲?汤愠扭潡牲慩瑮潥爠祦?牳敨愠牳数摥?潩捥瑳漠灩畮猠敓獯???楥??漠畓牰湡慩汮?漠昼??湒癥散牥瑮整戠牁慤瑶敡?偣慥瑳栠潩汮漠杆祩??椠??????????????????????戱爮?孢??嵛′?畝挠歅?????佉癁敍爬猠瑄牥潲海?丠???倠慉瑳瑭潡湩??坍???湬搭敌牡獭潩湥??????潳牳穯敵汫慩渠祁??????慨捥瑳敨牴楡慷?愠獈猬漠捂楡慹瑯敵摭?眠楅瑍栮?獍瑯牬慥湣摵敬摡?挠敡瑮慤挠数慨湥獮?晴特潰浩?琠档敨?湲潡牣瑴桥敲慩獺瑡?啩卯??慯湦搠?獩漾畐瑨桯睴敯獢瑡??汥潲物極摭愼??甾氠晤?捭潳慥獬瑡獥???楯??椠獳敯慭獥攠獭?潲晩??焠畦慩瑳楨捥?传物杮愠湌楡獫浥猠??業??????????っ?????????????扥牮?孳??崼??猾攬渠戲攰爱朸?????债愳琱栵漭朳攲渲椮挼楢瑲社?愲渶摝?癔楡牯甠汚攬渠捓敨???楃??汚楨湯極挠慓氬??楡据牧漠扎椬漠汗潡杮祧?則敌瘬椠敗睡獮??楙?????????ㄠ?????ぴ?????戠牯?嬠??崾??慯瑴慯湢穡慣?塥???佭猠潤牡業潳??剡??启物愾渠獳捵牢楳灰琮漠洼敩￣捤桡慭湳来敬獡?椼港?爾攠獩灮潦湥獣整?瑯潮?瑩敮洠灣敵牬慴瑵畲牥敤?楳湩?瑶桥敲?晰楯獭桦?灥慴琠格潩朾敐湡??極?倠桡潲瑧潥扮慴捥瑵敳爼椯畩派?摩慮洠獅敡汳慴敥??椠??獩畮扡献瀠???楁?摵慡浣獵敬汴慵敲??椯??挬氠甲攰猱?琺漲‰由渭搲攰爵献琼慢湲搾?琲样敝?敚浨敡牮杧攠湚挬攠?潵映?搬椠獗敡慮獧攠?漬甠瑗扡牮敧愠歙獇?愠瑊?楡湮捧爠教愬猠敌摩?獯攠慍睊愬琠敒牯?瑧攠浘灊攮爠慆瑩畲牳整猠???楯?側?潯卦?佳乫???極????ち????ㄠ?????? by Photobacterium damselae subsp. damselae in net-cage cultured black rockfish (Sebastes schlegeli). Aquaculture, 2019:1-7.
    [28] Shao P, Yong P, Zhou W, Sun JH, Wang YZ, Tang QP, Ren ST, Wu Z, ZhaoCY, Xu YF. First isolation of Photobacterium damselae subsp. damselae from half-smooth tongue sole suffering from skin-ulceration disease. Aquaculture (Amsterdam, Netherlands), 511:734208-734208.
    [29] Chaban B, Hughes HV, Beeby M. The flagellum in bacterial pathogens:for motility and a whole lot more. Seminars in Cell & Developmental Biology, 2015, 46:91-103.
    [30] Osorio CR, Vences A, Matanza XM, Terceti MS. Photobacterium damselae subsp. damselae, a generalist pathogen with unique virulence factors and high genetic diversity. Journal of Bacteriology, 2018, 200(15).
    [31] 邓益琴, 徐力文, 张亚秋, 郭志勋, 冯娟. 我国华南沿海海水养殖鱼类病原菌美人鱼发光杆菌(Photobacterium damselae)分离株的毒力基因和耐药性分析. 微生物学报, 2020, 60(11):2606-2619. (in Chinese) Deng YQ, Xu LW, Zhang YQ, Guo ZX, Feng J. Analysis of virulence genes and antibiotic resistance of Photobacterium damselae isolated from marine fishes in coastal South China. Acta
    Related
    Cited by
    Comments
    Comments
    分享到微博
    Submit
Get Citation

Xiao Liu, Zheng Zhang, Lifang Wang, Yongxiang Yu, Yingeng Wang, Meijie Liao, Guosi Xie, Jing Xing, Hao Zhang, Kai Wang, Yue Xu. Phenotypic and genetic diversity of Photobacterium damsela subsp. damsela isolated from aquatic animals in Hainan Province and Bohai Sea region. [J]. Acta Microbiologica Sinica, 2021, 61(7): 2101-2111

Copy
Share
Article Metrics
  • Abstract:320
  • PDF: 1183
  • HTML: 1526
  • Cited by: 0
History
  • Received:September 18,2020
  • Revised:January 21,2021
  • Online: July 07,2021
Article QR Code
  • WeChat ID
  • Mobile Terminal

Acta Microbiologica Sinica ® 2025 All Rights Reserved