Genome sequencing and bioinformatics analysis of cellulolytic fungus Aspergillus fumigatus HZ1

Abstract:

[Objective] Aspergillus fumigatus is a cellulolytic fungus. The research of its genome will facilitate the mining and development of enzyme resources associated with cellulose degradation. [Methods] A. fumigatus HZ1 was isolated from the gut of Cyrtotrachelus buqueti by CMC selective medium and Congo red staining method. Illumina PE50 platform was used to conduct genome sequence and the relative bioinformatics analysis was performed subsequently. Furthermore, the cellulase activities of this strain were determined by DNS method. [Results] The genome size of A. fumigatus HZ1 was 27.45 Mb with a GC content of 49.43%. The results from NR, KOG, GO, Swissprot, eggNOG, KEGG and Pfam database annotations reveal that the genome contained 9473 genes, and the carbohydrate active enzyme (CAZyme) annotation shows that the genome contained 534 CAZyme genes and coincided with the distribution of CAZyme in the other four A. fumigatus genomes. A variety of cellulase genes, hemicellulase genes and ligninase genes related to lignocellulose degradation were identified. In addition, cellulase activity showed an increasing trend and high activity in CMC medium. [Conclusion] Our findings provide reference for the application of A. fumigatus.