Analysis on characteristics of microbiome structure in non-small cell lung cancer patients

Abstract:

[Objective] To describe and compare the structure of intestinal microbiota between patients with non-small cell lung cancer and benign lung disease and explore the influence of specific microbial flora in the lung cancer.[Methods] We collected 63 cases of fecal samples which including 39 non-small cell lung cancer patients and 24 benign lung disease patients and use high-throughput second-generation sequencing technology platform to analyze the V3-V4 region of 16S rDNA gene in fecal samples of bacteria to explore the overall structural characteristic of intestinal microbiota.[Results] Lachnospira, Ruminococcus, Faecalibacterium, Roseburia, Fusicatenibacter are significantly enriched in lung adenocarcinoma patients. But in lung squamous cell carcinoma and benign lesions there is no obvious difference. And Haemophilus, Prevotella, Streptococcus, Citrobacter are significantly enriched in the intestines of patients with benign diseases.[Conclusions] Non-small cell lung cancer and benign diseases have different gut microbiome.

Key words:non-small cell lung cancer;gut microbiome;16S rDNA sequencing;differential analysis;tumor microecology