Xiaolong Li
College of Resources and Environment Sciences, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, ChinaYifei Wu
College of Resources and Environment Sciences, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, ChinaJun Zhang
College of Resources and Environment Sciences, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China[Objective] The purpose of this study is to isolate and identify the MAs(III) demethylation strains. We cloned and expressed the arsI gene involved in MAs(III)-demethylation in Bacillus aryabhattai FJ-6 and characterized the ArsI protein.[Methods] The arsI gene fragment (432 bp) was amplified with PCR. Recombinant plasmid pET29a-arsI was constructed and transformed into Escherichia coli BL21(DE3) for heterologous expression, and the expressed protein was detected with SDS-PAGE. The enzyme activity of ArsI was determined by using HPLC-ICP-MS.[Results] The arsI gene was cloned and the recombinant plasmid pET29a-arsI was expressed. The molecular weight of the recombinant protein was 17.4 kDa.[Conclusion]] The activity of ArsI protein with MAs(III) demethylation was clarified.
Xiaolong Li, Yifei Wu, Jun Zhang. Identification of an methylarsenite demethylation bacterium Bacillus aryabhattai FJ-6 and characterization of methylarsenite demethylation gene. [J]. Acta Microbiologica Sinica, 2021, 61(10): 3149-3158
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