Diversity of gut microbiota in Tadorna ferruginea living in different habitats of Xinjiang province

Abstract:

[Objective] Explore the diversity of gut microbiota of Tadorna ferruginea and its relationship with associated environment.[Methods] The fresh fecal samples of Tadorna ferruginea were randomly collected from five habitats at the northern Tianshan Mountain in the autumn of 2017, V3-V4 region was amplified with bacterial 16S rDNA universal primer, and high-throughput sequencing was performed, and then the software and online tools including BLAST, USEARCH and QIIME were used to analyze the bacterial diversity and compare the differences among the samples from different habitats.[Results] A total of 408 036 valid sequences and 1 014 OTUs were sequenced. The bacteria from the samples belonged to 25 phylum and 397 genera. At phylum level Proteobacteria and Firmicutes were dominant, meanwhile Bacteroidetes, Fusobacteria, Actinobacteria, Cyanobacteria, Spirochaetes, Saccharibacteria and Elusimicrobia are also abundance in the detected fecal samples. However, the proportions of these bacteria were diverse in these five habitat sites. In addition, the bacterial community of each site was significantly different at genus level. Cetobacterium, Megamonas, Pseudomonas, Lactobacillus and Streptococcus were the dominant bacteria in the samples from Aibi Lake, Anjihai reservoir, Kuitun reservoir, Moguhu reservoir and Bayi reservoir, respectively. Correlation analysis showed that the gut bacterial community was greatly influenced by the environment and food.[Conclusion] The diversity of gut microbiota of the Tadorna ferruginea in these five habitats in Xinjiang province was abundant, and there were some similarity and also specificity of the bacterial community in different sites, which were mainly related to the environment and food compositions in the habitat for Tadorna ferruginea.

Key words:Tadorna ferruginea;gut microbiota;diversity;high-throughput sequencing;habitat