Toxicological evaluation of Corallococcus sp. strain EGB and its ferments

Abstract:

[Objective] We evaluated the safety of Corallococcus sp. strain EGB to provide a scientific basis for the development of novel biocontrol agent and its safe application to the environment.[Methods] The genotoxicity of the cells and fermentation products of strain EGB were investigated by Ames test, polychromatic erythrocyte micronucleus test, and testis spermatocyte chromosome aberration assay in mice. Intragastric administration was employed to study the acute toxicity and 28-d subchronic toxicity.[Results] The results of Ames test, polychromatic erythrocyte micronucleus test, and testis spermatocyte chromosome aberration assay in mice revealed that the cells and fermentation products of strain EGB exhibited no genotoxic potential within the testing system. The LD₅₀ values of the cells and fermentation products of strain EGB were greater than 10 g/kg BW in ICR mice. In the 28 days subchronic toxicity test, the body weight, food and water consumption, blood biochemical indexes, blood routine parameters, organ weights, and histopathology of treatment groups showed no difference compared with those of the control group (P<0.05).[Conclusion] Strain EGB and its ferments could be classified as no toxicity and are safe for practical application in the plant disease control and bioconversion.

Key words:Corallococcus sp. strain EGB;ferments;safety evaluation;genotoxicity;subchronic toxicity