LU Weilai
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, ChinaXU Tianshun
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China;School of Life Sciences, Hebei University, Baoding 071002, Hebei, ChinaTANG Hui
School of Life Sciences, Hebei University, Baoding 071002, Hebei, ChinaJIANG Jingkun
School of Environment, Tsinghua University, Beijing 100084, ChinaFU Yu
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China;Savaid Medical School, University of Chinese Academy of Sciences, Beijing 100049, ChinaThe bioaerosol harbors diverse microorganisms.The sampling of microbial aerosols and the identification of microorganisms in aerosols are the key to understanding the biological characteristics of microbial aerosols,which is essential for preventing bioaerosol-mediated pathogen transmission.This article briefly reviewed the biological characteristics and potential hazards of microbial aerosols and introduced the general methods for microbial aerosol sampling with respect to their performance,operating mechanisms,advantages,and limitations,in an aim to assist the design of best practices for bioaerosol sampling.Three major (culture-dependent,culture-independent,and single-cell) techniques for the identification of bacteria,fungi,and viruses in bioaerosols were expounded.Finally,the future techniques for microbial aerosol collection and microbial identification were prospected.The cross-disciplinary interaction will facilitate the development of novel methods for bioaerosol sampling and detection.With this review,we hope to provide a theoretical basis for the development of sampling methods and comprehensive characterization of bioaerosols.
LU Weilai, XU Tianshun, TANG Hui, JIANG Jingkun, FU Yu. Technologies for microbial aerosol sampling and identification:a review and current perspective. [J]. Acta Microbiologica Sinica, 2022, 62(4): 1345-1361
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