Technologies for microbial aerosol sampling and identification:a review and current perspective
Author:
  • Article
  • | |
  • Metrics
  • |
  • Reference [100]
  • |
  • Related
  • |
  • Cited by
  • | |
  • Comments
    Abstract:

    The bioaerosol harbors diverse microorganisms.The sampling of microbial aerosols and the identification of microorganisms in aerosols are the key to understanding the biological characteristics of microbial aerosols,which is essential for preventing bioaerosol-mediated pathogen transmission.This article briefly reviewed the biological characteristics and potential hazards of microbial aerosols and introduced the general methods for microbial aerosol sampling with respect to their performance,operating mechanisms,advantages,and limitations,in an aim to assist the design of best practices for bioaerosol sampling.Three major (culture-dependent,culture-independent,and single-cell) techniques for the identification of bacteria,fungi,and viruses in bioaerosols were expounded.Finally,the future techniques for microbial aerosol collection and microbial identification were prospected.The cross-disciplinary interaction will facilitate the development of novel methods for bioaerosol sampling and detection.With this review,we hope to provide a theoretical basis for the development of sampling methods and comprehensive characterization of bioaerosols.

    Reference
    [1] Després V, Huffman JA, Burrows SM, Hoose C, Safatov A, Buryak G, Fröhlich-Nowoisky J, Elbert W, Andreae M, Pöschl U, Jaenicke R. Primary biological aerosol particles in the atmosphere:a review. Tellus B:Chemical and Physical Meteorology, 2012, 64(1):15598.
    [2] Xu ZQ, Wu Y, Shen FX, Chen Q, Tan MM, Yao MS. Bioaerosol science, technology, and engineering:past, present, and future. Aerosol Science and Technology, 2011, 45(11):1337-1349.
    [3] Gong J, Qi JH, E BB, Yin YD, Gao DM. Concentration, viability and size distribution of bacteria in atmospheric bioaerosols under different types of pollution. Environmental Pollution, 2020, 257:113485.
    [4] Šantl-Temkiv T, Sikoparija B, Maki T, Carotenuto F, Amato P, Yao MS, Morris CE, Schnell R, Jaenicke R, Pöhlker C, DeMott PJ, Hill TCJ, Huffman JA. Bioaerosol field measurements:challenges and perspectives in outdoor studies. Aerosol Science and Technology, 2020, 54(5):520-546.
    [5] Tignat-Perrier R, Dommergue A, Thollot A, Keuschnig C, Magand O, Vogel TM, Larose C. Global airborne microbial communities controlled by surrounding landscapes and wind conditions. Scientific Reports, 2019, 9:14441.
    [6] Stetzenbach LD. Airborne infectious microorganisms. Encyclopedia of Microbiology. Amsterdam:Elsevier, 2009:175-182.
    [7] Li YP, Lu R, Li WX, Xie ZS, Song Y. Concentrations and size distributions of viable bioaerosols under various weather conditions in a typical semi-arid city of Northwest China. Journal of Aerosol Science, 2017, 106:83-92.
    [8] Liu H, Zhang X, Zhang H, Yao XW, Zhou M, Wang JQ, He ZF, Zhang HH, Lou LP, Mao WH, Zheng P, Hu BL. Effect of air pollution on the total bacteria and pathogenic bacteria in different sizes of particulate matter. Environmental Pollution, 2018, 233:483-493.
    [9] Cao C, Jiang WJ, Wang BY, Fang JH, Lang JD, Tian G, Jiang JK, Zhu TF. Inhalable microorganisms in Beijing's PM2.5 and PM10 pollutants during a severe smog event. Environmental Science& Technology, 2014, 48(3):1499-1507.
    [10] Douwes J, Eduard W, Thorne PS. Bioaerosols. International Encyclopedia of Public Health. Amsterdam:Elsevier, 2017:210-218.
    [11] Kim KH, Kabir E, Jahan SA. Airborne bioaerosols and their impact on human health. Journal of Environmental Sciences, 2018, 67:23-35.
    [12] Jiang JK, Vincent Fu Y, Liu L, Kulmala M. Transmission via aerosols:plausible differences among emerging coronaviruses. Aerosol Science and Technology, 2020, 54(8):865-868.
    [13] Mainelis G. Bioaerosol sampling:classical approaches, advances, and perspectives. Aerosol Science and Technology, 2020, 54(5):496-519.
    [14] Haddrell AE, Thomas RJ. Aerobiology:experimental considerations, observations, and future tools. Applied and Environmental Microbiology, 2017, 83(17):e0080917.
    [15] Mortazavi R, Ariya PA. The impact of renovation on indoor airborne bacterial and fungal populations. Indoor and Built Environment, 2017, 26(10):1351-1361.
    [16] Yazdanbakhsh A, Ghazi M, Sahlabadi F, Teimouri F. Data on airborne bacteria and fungi emission from a conventional hospital wastewater treatment plant. Data in Brief, 2020, 28:105019.
    [17] Pasquarella C, Vitali P, Saccani E, Manotti P, Boccuni C, Ugolotti M, Signorelli C, Mariotti F, Sansebastiano GE, Albertini R. Microbial air monitoring in operating theatres:experience at the University hospital of Parma. Journal of Hospital Infection, 2012, 81(1):50-57.
    [18] Grinshpun SA, Buttner MP, Mainelis G, Willeke K. Sampling for airborne microorganisms.//Yates MV, Nakatsu CH, Miller RV, Pillai SD. Manual of Environmental Microbiology. Washington, DC, USA:ASM Press, 2015:3.2.2-1-3.2.2-17.
    [19] Zheng YH, Yao MS. Liquid impinger BioSampler's performance for size-resolved viable bioaerosol particles. Journal of Aerosol Science, 2017, 106:34-42.
    [20] Haig CW, Mackay WG, Walker JT, Williams C. Bioaerosol sampling:sampling mechanisms, bioefficiency and field studies. Journal of Hospital Infection, 2016, 93(3):242-255.
    [21] Lim JH, Park D, Yook SJ. Development of a multi-slit virtual impactor as a high-volume bio-aerosol sampler. Separation and Purification Technology, 2020, 250:117275.
    [22] Chen HX, Yao MS. A high-flow portable biological aerosol trap (HighBioTrap) for rapid microbial detection. Journal of Aerosol Science, 2018, 117:212-223.
    [23] Griffin DW. Atmospheric movement of microorganisms in clouds of desert dust and implications for human health. Clinical Microbiology Reviews, 2007, 20(3):459-477, table of contents.
    [24] Misiulia D, Andersson AG, Lundström TS. Effects of the inlet angle on the collection efficiency of a cyclone with helical-roof inlet. Powder Technology, 2017, 305:48-55.
    [25] Brar LS, Sharma RP, Elsayed K. The effect of the cyclone length on the performance of Stairmand high-efficiency cyclone. Powder Technology, 2015, 286:668-677.
    [26] Sung G, Ahn C, Kulkarni A, Shin WG, Kim T. Highly efficient in-line wet cyclone air sampler for airborne virus detection. Journal of Mechanical Science and Technology, 2017, 31(9):4363-4369.
    [27] Sung G, Kim HU, Shin D, Shin WG, Kim T. High efficiency axial wet cyclone air sampler. Aerosol and Air Quality Research, 2018, 18(10):2529-2537.
    [28] Pan M, Lednicky JA, Wu CY. Collection, particle sizing and detection of airborne viruses. Journal of Applied Microbiology, 2019, 127(6):1596-1611.
    [29] Cho YS, Hong SC, Choi J, Jung JH. Development of an automated wet-cyclone system for rapid, continuous and enriched bioaerosol sampling and its application to real-time detection. Sensors and Actuators B:Chemical, 2019, 284:525-533.
    [30] Ferguson RMW, Garcia-Alcega S, Coulon F, Dumbrell AJ, Whitby C, Colbeck I. Bioaerosol biomonitoring:sampling optimization for molecular microbial ecology. Molecular Ecology Resources, 2019, 19(3):672-690.
    [31] Vestlund AT, Al-Ashaab R, Tyrrel SF, Longhurst PJ, Pollard SJT, Drew GH. Morphological classification of bioaerosols from composting using scanning electron microscopy. Waste Management, 2014, 34(7):1101-1108.
    [32] Chen LWA, Zhang M, Liu T, Fortier K, Chow JC, Alonzo F, Kolberg R, Cao JJ, Lin G, Patel TY, Cruz P, Buttner MP, Watson JG. Evaluation of epifluorescence methods for quantifying bioaerosols in fine and coarse particulate air pollution. Atmospheric Environment, 2019, 213:620-628.
    [33] Bekking C, Yip L, Groulx N, Doggett N, Finn M, Mubareka S. Evaluation of bioaerosol samplers for the detection and quantification of influenza virus from artificial aerosols and influenza virus-infected ferrets. Influenza and Other Respiratory Viruses, 2019, 13(6):564-573.
    [34] Wang CH, Chen BT, Han BC, Liu ACY, Hung PC, Chen CY, Chao HJ. Field evaluation of personal sampling methods for multiple bioaerosols. PLoS One, 2015, 10(3):e0120308.
    [35] Jeong SY, Kim TG. Comparison of five membrane filters to collect bioaerosols for airborne microbiome analysis. Journal of Applied Microbiology, 2021, 131(2):780-790.
    [36] Kim HR, An S, Hwang J. High air flow-rate electrostatic sampler for the rapid monitoring of airborne coronavirus and influenza viruses. Journal of Hazardous Materials, 2021, 412:125219.
    [37] Rufino De Sousa N, Sandström N, Shen L, Håkansson K, Vezozzo R, Udekwu KI, Croda J, Rothfuchs AG. A fieldable electrostatic air sampler enabling tuberculosis detection in bioaerosols. Tuberculosis, 2020, 120:101896.
    [38] Priyamvada H, Kumaragama K, Chrzan A, Athukorala C, Sur S, Dhaniyala S. Design and evaluation of a new electrostatic precipitation-based portable low-cost sampler for bioaerosol monitoring. Aerosol Science and Technology, 2021, 55(1):24-36.
    [39] Mescioglu E, Paytan A, Mitchell BW, Griffin DW. Efficiency of bioaerosol samplers:a comparison study. Aerobiologia, 2021, 37(3):447-459.
    [40] Han TT, Thomas NM, Mainelis G. Performance of personal electrostatic bioaerosol sampler (PEBS) when collecting airborne microorganisms. Journal of Aerosol Science, 2018, 124:54-67.
    [41] Piri AM, Kim HR, Park DH, Hwang J. Increased survivability of coronavirus and H1N1 influenza virus under electrostatic aerosol-to-hydrosol sampling. Journal of Hazardous Materials, 2021, 413:125417.
    [42] Nieto-Caballero M, Savage N, Keady P, Hernandez M. High fidelity recovery of airborne microbial genetic materials by direct condensation capture into genomic preservatives. Journal of Microbiological Methods, 2019, 157:1-3.
    [43] Pan M, Eiguren-Fernandez A, Hsieh H, Afshar-Mohajer N, Hering SV, Lednicky J, Fan ZH, Wu CY. Efficient collection of viable virus aerosol through laminar-flow, water-based condensational particle growth. Journal of Applied Microbiology, 2016, 120(3):805-815.
    [44] Lednicky J, Pan MH, Loeb J, Hsieh H, Eiguren-Fernandez A, Hering S, Fan ZH, Wu CY. Highly efficient collection of infectious pandemic influenza H1N1 virus (2009) through laminar-flow water based condensation. Aerosol Science and Technology, 2016, 50(7):i-iv.
    [45] Pan MH, Bonny TS, Loeb J, Jiang X, Lednicky JA, Eiguren-Fernandez A, Hering S, Fan ZH, Wu CY. Collection of viable aerosolized influenza virus and other respiratory viruses in a student health care center through water-based condensation growth. mSphere, 2017, 2(5):e0025117.
    [46] Degois J, Dubuis ME, Turgeon N, Veillette M, Duchaine C. Condensation sampler efficiency for the recovery and infectivity preservation of viral bioaerosols. Aerosol Science and Technology, 2021, 55(6):653-664.
    [47] Lednicky JA, Lauzardo M, Fan ZH, Jutla A, Tilly TB, Gangwar M, Usmani M, Shankar SN, Mohamed K, Eiguren-Fernandez A, Stephenson CJ, Alam MM, Elbadry MA, Loeb JC, Subramaniam K, Waltzek TB, Cherabuddi K, Morris JG Jr, Wu CY. Viable SARS-CoV-2 in the air of a hospital room with COVID-19 patients. International Journal of Infectious Diseases, 2020, 100:476-482.
    [48] Tsay MD, Tseng CC, Wu NX, Lai CY. Size distribution and antibiotic-resistant characteristics of bacterial bioaerosol in intensive care unit before and during visits to patients. Environment International, 2020, 144:106024.
    [49] Jalili D, Dehghani M, Fadaei A, Alimohammadi M. Assessment of airborne bacterial and fungal communities in shahrekord hospitals. Journal of Environmental and Public Health, 2021, 2021:8864051.
    [50] Kim MH, Baek KO, Park GG, Jang JY, Lee JH. A study on concentration, identification, and reduction of airborne microorganisms in the military working dog clinic. Safety and Health at Work, 2020, 11(4):517-525.
    [51] Pan YM, Ren QQ, Chen P, Wu JG, Wu ZD, Zhang GX. Insight into microbial community aerosols associated with electronic waste handling facilities by culture-dependent and culture-independent methods. Frontiers in Public Health, 2021, 9:657784.
    [52] Sivagnanasundaram P, Amarasekara RWK, Madegedara RMD, Ekanayake A, Magana-Arachchi DN. Assessment of airborne bacterial and fungal communities in selected areas of teaching hospital, Kandy, Sri Lanka. BioMed Research International, 2019, 2019:7393926.
    [53] Ling S, Hui L. Evaluation of the complexity of indoor air in hospital wards based on PM2.5, real-time PCR, adenosine triphosphate bioluminescence assay, microbial culture and mass spectrometry. BMC Infectious Diseases, 2019, 19(1):646.
    [54] Jäckel U, Martin E, Schäfer J. Heterogeneity in cultivation-based monitoring of airborne bacterial biodiversity in animal farms. Annals of Work Exposures and Health, 2017, 61(6):643-655.
    [55] Hu W, Murata K, Fan CL, Huang S, Matsusaki H, Fu PQ, Zhang DZ. Abundance and viability of particle-attached and free-floating bacteria in dusty and nondusty air. Biogeosciences, 2020, 17(17):4477-4487.
    [56] Tham KW, Zuraimi MS. Size relationship between airborne viable bacteria and particles in a controlled indoor environment study. Indoor Air, 2005, 15(Suppl 9):48-57.
    [57] Nehme B, Létourneau V, Forster RJ, Veillette M, Duchaine C. Culture-independent approach of the bacterial bioaerosol diversity in the standard swine confinement buildings, and assessment of the seasonal effect. Environmental Microbiology, 2008, 10(3):665-675.
    [58] Riccardi C, Di Filippo P, Pomata D, Simonetti G, Castellani F, Uccelletti D, Bruni E, Federici E, Buiarelli F. Comparison of analytical approaches for identifying airborne microorganisms in a livestock facility. Science of the Total Environment, 2021, 783:147044.
    [59] Wei DJ, Liu WT, Chin HT, Lin CH, Chen IC, Chang YT. An investigation of airborne bioaerosols and endotoxins present in indoor traditional wet markets before and after operation in Taiwan:a case study. International Journal of Environmental Research and Public Health, 2021, 18(6):2945.
    [60] Prass M, Andreae MO, De Araùjo AC, Artaxo P, Ditas F, Elbert W, Förster JD, Franco MA, Hrabe De Angelis I, Kesselmeier J, Klimach T, Kremper LA, Thines E, Walter D, Weber J, Weber B, Fuchs BM, Pöschl U, Pöhlker C. Bioaerosols in the Amazon rain forest:temporal variations and vertical profiles of eukarya, bacteria, and archaea. Biogeosciences, 2021, 18(17):4873-4887.
    [61] Anedda E, Carletto G, Gilli G, Traversi D. Monitoring of air microbial contaminations in different bioenergy facilities using cultural and biomolecular methods. International Journal of Environmental Research and Public Health, 2019, 16(14):2546.
    [62] Mu FF, Li YP, Lu R, Qi YZ, Xie WW, Bai WY. Source identification of airborne bacteria in the mountainous area and the urban areas. Atmospheric Research, 2020, 231:104676.
    [63] Ye J, Qian H, Zhang JS, Sun F, Zhuge Y, Zheng XH. Combining culturing and 16S rDNA sequencing to reveal seasonal and room variations of household airborne bacteria and correlative environmental factors in Nanjing, southeast China. Indoor Air, 2021, 31(4):1095-1108.
    [64] Li XY, Chen HX, Yao MS. Microbial emission levels and diversities from different land use types. Environment International, 2020, 143:105988.
    [65] Han YP, Yang T, Chen TZ, Li L, Liu JX. Characteristics of submicron aerosols produced during aeration in wastewater treatment. Science of the Total Environment, 2019, 696:134019.
    [66] Nygaard AB, Tunsjø HS, Meisal R, Charnock C. A preliminary study on the potential of nanopore MinION and Illumina MiSeq 16S rRNA gene sequencing to characterize building-dust microbiomes. Scientific Reports, 2020, 10:3209.
    [67] Galès A, Bru-Adan V, Godon JJ, Delabre K, Catala P, Ponthieux A, Chevallier M, Birot E, Steyer JP, Wéry N. Predominance of single bacterial cells in composting bioaerosols. Atmospheric Environment, 2015, 107:225-232.
    [68] Wang YF, Zhang XD, Yang N, Ma GX, Du XX, Mao HP. Separation-enrichment method for airborne disease spores based on microfluidic chip. International Journal of Agricultural and Biological Engineering, 2021, 14(5):199-205.
    [69] Xu PF, Zhang RB, Yang N, Kwabena Oppong P, Sun J, Wang P. High-precision extraction and concentration detection of airborne disease microorganisms based on microfluidic chip. Biomicrofluidics, 2019, 13(2):024110.
    [70] Damit B. Droplet-based microfluidics detector for bioaerosol detection. Aerosol Science and Technology, 2017, 51(4):488-500.
    [71] Choi J, Hong SC, Kim W, Jung JH. Highly enriched, controllable, continuous aerosol sampling using inertial microfluidics and its application to real-time detection of airborne bacteria. ACS Sensors, 2017, 2(4):513-521.
    [72] Cui L, Yang K, Li HZ, Zhang H, Su JQ, Paraskevaidi M, Martin FL, Ren B, Zhu YG. Functional single-cell approach to probing nitrogen-fixing bacteria in soil communities by resonance Raman spectroscopy with 15N2 labeling. Analytical Chemistry, 2018, 90(8):5082-5089.
    [73] Lu WL, Chen XQ, Wang L, Li HF, Fu YV. Combination of an artificial intelligence approach and laser tweezers Raman spectroscopy for microbial identification. Analytical Chemistry, 2020, 92(9):6288-6296.
    [74] Yu SX, Li HF, Li X, Fu YV, Liu FH. Classification of pathogens by Raman spectroscopy combined with generative adversarial networks. Science of the Total Environment, 2020, 726:138477.
    [75] Heidari Baladehi M, Hekmatara M, He YH, Bhaskar Y, Wang ZB, Liu L, Ji YT, Xu J. Culture-free identification and metabolic profiling of microalgal single cells via ensemble learning of ramanomes. Analytical Chemistry, 2021, 93(25):8872-8880.
    [76] Choi J, Lee J, Jung JH. Fully integrated optofluidic SERS platform for real-time and continuous characterization of airborne microorganisms. Biosensors and Bioelectronics, 2020, 169:112611.
    [77] Ai YK, Alali H, Pan YL, Videen G, Wang CJ. Single-particle optical-trapping Raman spectroscopy for the detection and identification of aerosolized airborne biological particles. Measurement Science and Technology, 2021, 32(5):055207.
    [78] Tahir MA, Zhang XL, Cheng HY, Xu D, Feng YQ, Sui GD, Fu HB, Valev VK, Zhang LW, Chen JM. Klarite as a label-free SERS-based assay:a promising approach for atmospheric bioaerosol detection. The Analyst, 2019, 145(1):277-285.
    [79] Scoizec A, Niqueux E, Thomas R, Daniel P, Schmitz A, Le Bouquin S. Airborne detection of H5N8 highly pathogenic avian influenza virus genome in poultry farms, France. Frontiers in Veterinary Science, 2018, 5:15.
    [80] Coleman KK, Sigler WV. Airborne influenza a virus exposure in an elementary school. Scientific Reports, 2020, 10:1859.
    [81] Chamseddine A, Soudani N, Kanafani Z, Alameddine I, Dbaibo G, Zaraket H, El-Fadel M. Detection of influenza virus in air samples of patient rooms. Journal of Hospital Infection, 2021, 108:33-42.
    [82] Fulci V, Carissimi C, Laudadio I. COVID-19 and preparing for future ecological crises:hopes from metagenomics in facing current and future viral pandemic challenges. Omics:a Journal of Integrative Biology, 2021, 25(6):336-341.
    [83] Prussin Ⅱ AJ, Torres PJ, Shimashita J, Head SR, Bibby KJ, Kelley ST, Marr LC. Seasonal dynamics of DNA and RNA viral bioaerosol communities in a daycare center. Microbiome, 2019, 7(1):53.
    [84] Brisebois E, Veillette M, Dion-Dupont V, Lavoie J, Corbeil J, Culley A, Duchaine C. Human viral pathogens are pervasive in wastewater treatment center aerosols. Journal of Environmental Sciences, 2018, 67:45-53.
    [85] Morawska L, Milton DK. It is time to address airborne transmission of coronavirus disease 2019(COVID-19). Clinical Infectious Diseases, 2020, 71(9):2311-2313.
    [86] Wilson NM, Norton A, Young FP, Collins DW. Airborne transmission of severe acute respiratory syndrome coronavirus-2 to healthcare workers:a narrative review. Anaesthesia, 2020, 75(8):1086-1095.
    [87] Tang S, Mao YX, Jones RM, Tan QY, Ji JS, Li N, Shen J, Lv YB, Pan LJ, Ding P, Wang XC, Wang YB, MacIntyre CR, Shi XM. Aerosol transmission of SARS-CoV-2?Evidence, prevention and control. Environment International, 2020, 144:106039.
    [88] Kenarkoohi A, Noorimotlagh Z, Falahi S, Amarloei A, Mirzaee SA, Pakzad I, Bastani E. Hospital indoor air quality monitoring for the detection of SARS-CoV-2(COVID-19) virus. Science of the Total Environment, 2020, 748:141324.
    [89] Wang CC, Prather KA, Sznitman J, Jimenez JL, Lakdawala SS, Tufekci Z, Marr LC. Airborne transmission of respiratory viruses. Science, 2021, 373(6558):eabd9149.
    [90] Liu Y, Ning Z, Chen Y, Guo M, Liu YL, Gali NK, Sun L, Duan YS, Cai J, Westerdahl D, Liu XJ, Xu K, Ho KF, Kan HD, Fu QY, Lan K. Aerodynamic analysis of SARS-CoV-2 in two Wuhan hospitals. Nature, 2020, 582(7813):557-560.
    [91] Van Doremalen N, Bushmaker T, Morris DH, Holbrook MG, Gamble A, Williamson BN, Tamin A, Harcourt JL, Thornburg NJ, Gerber SI, Lloyd-Smith JO, De Wit E, Munster VJ. Aerosol and surface stability of SARS-CoV-2 as compared with SARS-CoV-1. The New England Journal of Medicine, 2020, 382(16):1564-1567.
    [92] Li YG, Qian H, Hang J, Chen XG, Cheng P, Ling H, Wang SQ, Liang P, Li JS, Xiao SL, Wei JJ, Liu L, Cowling BJ, Kang M. Probable airborne transmission of SARS-CoV-2 in a poorly ventilated restaurant. Building and Environment, 2021, 196:107788.
    [93] Lane MA, Brownsword EA, Babiker A, Ingersoll JM, Waggoner J, Ayers M, Klopman M, Uyeki TM, Lindsley WG, Kraft CS. Bioaerosol sampling for severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) in a referral center with critically ill coronavirus disease 2019(COVID-19) patients March-May 2020. Clinical Infectious Diseases, 2021, 73(7):e1790-e1794.
    [94] Borges JT, Nakada LYK, Maniero MG, Guimarães JR. SARS-CoV-2:a systematic review of indoor air sampling for virus detection. Environmental Science and Pollution Research International, 2021, 28(30):40460-40473.
    [95] Robotto A, Quaglino P, Lembo D, Morello M, Brizio E, Bardi L, Civra A. SARS-CoV-2 and indoor/outdoor air samples:a methodological approach to have consistent and comparable results. Environmental Research, 2021, 195:110847.
    [96] Shin J, Kim HR, Bae PK, Yoo H, Kim J, Choi Y, Kang A, Yun WS, Shin YB, Hwang J, Hong S. Reusable surface amplified nanobiosensor for the sub PFU/mL level detection of airborne virus. Scientific Reports, 2021, 11:16776.
    [97] Bhardwaj J, Kim MW, Jang J. Rapid airborne influenza virus quantification using an antibody-based electrochemical paper sensor and electrostatic particle concentrator. Environmental Science& Technology, 2020, 54(17):10700-10712.
    [98] Jiang X, Loeb JC, Pan MH, Tilly TB, Eiguren-Fernandez A, Lednicky JA, Wu CY, Fan ZH. Integration of sample preparation with RNA-amplification in a hand-held device for airborne virus detection. Analytica Chimica Acta, 2021, 1165:338542.
    [99] Lee I, Seok Y, Jung H, Yang B, Lee J, Kim J, Pyo H, Song CS, Choi W, Kim MG, Lee J. Integrated bioaerosol sampling/monitoring platform:field-deployable and rapid detection of airborne viruses. ACS Sensors, 2020, 5(12):3915-3922.
    [100] Li MX, Wang L, Qi WZ, Liu YJ, Lin JH. Challenges and perspectives for biosensing of bioaerosol containing pathogenic microorganisms. Micromachines, 2021, 12(7):798.
    Related
    Cited by
    Comments
    Comments
    分享到微博
    Submit
Get Citation

LU Weilai, XU Tianshun, TANG Hui, JIANG Jingkun, FU Yu. Technologies for microbial aerosol sampling and identification:a review and current perspective. [J]. Acta Microbiologica Sinica, 2022, 62(4): 1345-1361

Copy
Share
Article Metrics
  • Abstract:695
  • PDF: 1778
  • HTML: 6141
  • Cited by: 0
History
  • Received:August 31,2021
  • Revised:December 09,2021
  • Online: April 15,2022
Article QR Code