Research progress of detection technology of Helicobacter pylori and beyond

doi:10.13343/j.cnki.wsxb.20210640

Home > Archive>Volume 62, Issue 5, 2022 >1645-1655. DOI:10.13343/j.cnki.wsxb.20210640

Research progress of detection technology of Helicobacter pylori and beyond
DOI:
                        10.13343/j.cnki.wsxb.20210640
                    
CSTR:
                        32112.14.j.AMS.20210640
                    
Author:
                        LI YaqianLI Yaqian
College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, Jiangsu, China;Important Disease Animal and Zoonosis Prevention and Control Collaborative Innovation Center of Jiangsu Province, Yangzhou 225009, Jiangsu, China;Joint Laboratory of International Cooperation on Agriculture and Agricultural Product Safety of Ministry of Education and Joint Laboratory of International Cooperation on Prevention and Control Technology of Important Animal Diseases and Zoonoses of Jiangsu, Yangzhou 225009, Jiangsu, China
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LIU JiaqiLIU Jiaqi
College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, Jiangsu, China;Important Disease Animal and Zoonosis Prevention and Control Collaborative Innovation Center of Jiangsu Province, Yangzhou 225009, Jiangsu, China;Joint Laboratory of International Cooperation on Agriculture and Agricultural Product Safety of Ministry of Education and Joint Laboratory of International Cooperation on Prevention and Control Technology of Important Animal Diseases and Zoonoses of Jiangsu, Yangzhou 225009, Jiangsu, China
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ZHU XiaofangZHU Xiaofang
Department of Dermatology, College of Clinical Medicine of Yangzhou University, Yangzhou 225009, Jiangsu, China
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DUAN QiangdeDUAN Qiangde
College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, Jiangsu, China;Important Disease Animal and Zoonosis Prevention and Control Collaborative Innovation Center of Jiangsu Province, Yangzhou 225009, Jiangsu, China;Joint Laboratory of International Cooperation on Agriculture and Agricultural Product Safety of Ministry of Education and Joint Laboratory of International Cooperation on Prevention and Control Technology of Important Animal Diseases and Zoonoses of Jiangsu, Yangzhou 225009, Jiangsu, China
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ZHU GuoqiangZHU Guoqiang
College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, Jiangsu, China;Important Disease Animal and Zoonosis Prevention and Control Collaborative Innovation Center of Jiangsu Province, Yangzhou 225009, Jiangsu, China;Joint Laboratory of International Cooperation on Agriculture and Agricultural Product Safety of Ministry of Education and Joint Laboratory of International Cooperation on Prevention and Control Technology of Important Animal Diseases and Zoonoses of Jiangsu, Yangzhou 225009, Jiangsu, China
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Abstract:

Helicobacter pylori induces the incidence and development of many human gastrointestinal diseases via various virulence factors. Due to the lack of commercial vaccine and multi-drug resistance, it is difficult to eradicate this pathogen. Therefore, accurate detection technology is the key to prevent H.pylori infection and an important means of evaluating the treatment effect. The detection methods mainly include urea breath test, rapid urease test, stool antigen test, serological test, endoscopy, histopathologic examination, polymerase chain reaction, and bacteriological culture, each having specific strengths and weaknesses in clinical application. In the absence of an agreed-upon “gold standard” forH.pylori detection, we reviewed H.pylori detection techniques, analyzing their advantages and limitations. Particularly, we summarized the advantages of accurate, rapid, and convenient detection techniques in epidemiological investigation, aiming to provide a scientific reference for clinical application of and research on them.

Key words:Helicobacter pylori;infection;detection

Reference

[1] Boquet P, Ricci V. Intoxication strategy of Helicobacter pylori VacA toxin. Trends in Microbiology, 2012, 20(4):165-174.

[2] 涂宏飞,费素娟.幽门螺杆菌CagA及VacA致病机制的研究进展.中国中西医结合消化杂志, 2019, 27(5):399-402. Tu HF, Fei SJ. Research progress on pathogenesis of Helicobacter pylori CagA and VacA. Chinese Journal of Integrated Traditional and Western Medicine on Digestion, 2019, 27(5):399-402.(in Chinese)

[3] Knorr J, Ricci V, Hatakeyama M, Backert S. Classification of Helicobacter pylori virulence factors:is CagA a toxin or not?Trends in Microbiology, 2019, 27(9):731-738.

[4] Kaplan-Türköz B, Jiménez-Soto LF, Dian C, Ertl C, Remaut H, Louche A, Tosi T, Haas R, Terradot L. Structural insights into Helicobacter pylori oncoprotein CagA interaction with β1 integrin. Proceedings of the National Academy of Sciences of the United States of America, 2012, 109(36):14640-14645.

[5] Szabò I, Brutsche S, Tombola F, Moschioni M, Satin B, Telford JL, Rappuoli R, Montecucco C, Papini E, Zoratti M. Formation of anion-selective channels in the cell plasma membrane by the toxin VacA of Helicobacter pylori is required for its biological activity. The EMBO Journal, 1999, 18(20):5517-5527.

[6] Galmiche A, Rassow J, Doye A, Cagnol S, Chambard JC, Contamin S, De Thillot V, Just I, Ricci V, Solcia E, Van Obberghen E, Boquet P. The N-terminal 34 kDa fragment of Helicobacter pylori vacuolating cytotoxin targets mitochondria and induces cytochrome c release. The EMBO Journal, 2000, 19(23):6361-6370.

[7] Torres VJ, Van Compernolle SE, Sundrud MS, Unutmaz D, Cover TL. Helicobacter pylori vacuolating cytotoxin inhibits activation-induced proliferation of human T and B lymphocyte subsets. Journal of Immunology, 2007, 179(8):5433-5440.

[8] Oldani A, Cormont M, Hofman V, Chiozzi V, Oregioni O, Canonici A, Sciullo A, Sommi P, Fabbri A, Ricci V, Boquet P. Helicobacter pylori counteracts the apoptotic action of its VacA toxin by injecting the CagA protein into gastric epithelial cells. PLoS Pathogens, 2009, 5(10):e1000603.

[9] 袁世梅,杨伟,魏进武,廖俐雅,余登琼,左崇宇,余晓辉,宋仁,熊中政.不同诊断方法对消化内科幽门螺杆菌感染的诊断价值.检验医学与临床, 2021, 18(13):1948-1951. Yuan SM, Yang W, Wei JW, Liao LY, Yu DQ, Zuo CY, Yu XH, Song R, Xiong ZZ. Diagnostic value of different diagnostic methods for Helicobacter pylori infection in gastroenterology department. Laboratory Medicine and Clinic, 2021, 18(13):1948-1951.(in Chinese)

[10] Sugano K, Tack J, Kuipers EJ, Graham DY, El-Omar EM, Miura S, Haruma K, Asaka M, Uemura N, Malfertheiner P. Kyoto global consensus report on Helicobacter pylori gastritis.Gut, 2015, 64(9):1353-1367.

[11] 许玉春.慢性萎缩性胃炎患者幽门螺杆菌根除治疗的临床意义.山东大学硕士学位论文, 2020.

[12] Deng L, He XY, Tang B, Xiang Y, Yue JJ. An improved quantitative real-time polymerase chain reaction technology for Helicobacter pylori detection in stomach tissue and its application value in clinical precision testing. BMC Biotechnology, 2020, 20(1):33.

[13] Saracino IM, Zullo A, Holton J, Castelli V, Fiorini G, Zaccaro C, Ridola L, Ricci C, Gatta L, Vaira D. High prevalence of primary antibiotic resistance in Helicobacter pylori isolates in Italy. Journal of Gastrointestinal and Liver Diseases, 2012, 21(4):363-365.

[14] Zhang YX, Zhou LY, Song ZQ, Zhang JZ, He LH, Ding Y. Primary antibiotic resistance of Helicobacter pylori strains isolated from patients with dyspeptic symptoms in Beijing:a prospective serial study. World Journal of Gastroenterology, 2015, 21(9):2786-2792.

[15] 姚敏,李艳梅.人群中幽门螺杆菌感染现状及危险因素分析.世界最新医学信息文摘, 2018, 18(55):85-86. Yao M, Li YM. Analysis of Helicobacter pylori infection status and risk factors in the human population. World Latest Medicine Information, 2018, 18(55):85-86.(in Chinese)

[16] Kouitcheu Mabeku LB, Bello Epesse M, Fotsing S, Kamgang R, Tchidjo M. Stool antigen testing, a reliable noninvasive method of assessment of Helicobacter pylori infection among patients with gastro-duodenal disorders in Cameroon. Digestive Diseases and Sciences, 2021, 66(2):511-520.

[17] 李莉.幽门螺杆菌应用¹³C尿素呼气试验与¹⁴C尿素呼气试验检测的比较分析.中外医学研究, 2017, 15(36):92-94. Li L. Comparative analysis of ¹³C urea breath test and ¹⁴C urea breath test for detection of Helicobacter pylori. Chinese and Foreign Medical Research, 2017, 15(36):92-94.(in Chinese)

[18] Dondi E, Rapa A, Boldorini R, Fonio P, Zanetta S, Oderda G. High accuracy of noninvasive tests to diagnose Helicobacter pylori infection in very young children. The Journal of Pediatrics, 2006, 149(6):817-821.

[19] 周芳菲.幽门螺杆菌测试仪¹⁴C尿素呼气试验在幽门螺杆菌感染检测中的应用价值.中国实用医药, 2021, 16(23):53-55. Zhou FF. Application value of ¹⁴C urea breath test in the detection of Helicobacter pylori. China Practical Medicine, 2021, 16(23):53-55.(in Chinese)

[20] Lee TH, Lin CC, Chung CS, Lin CK, Liang CC, Tsai KC. Increasing biopsy number and sampling from gastric body improve the sensitivity of rapid urease test in patients with peptic ulcer bleeding. Digestive Diseases and Sciences, 2015, 60(2):454-457.

[21] 张少聪,陈卫刚,王凯莉,陈凯丽,郑勇. ¹⁴C-尿素呼气试验检测值与幽门螺杆菌感染密度的关系.吉林医学, 2021, 42(2):368-371. Zhang SC, Chen WG, Wang KL, Chen KL, Zheng Y. Relationship between C-14 breath test value and Helicobacter pylori infection density. Jilin Medical Journal, 2021, 42(2):368-371.(in Chinese)

[22] 曾妙,杨三三,李雪诺,陈安海.幽门螺旋杆菌的检测方法研究现状.海南医学, 2020, 31(6):784-788. Zeng M, Yang SS, Li XN, Chen AH. Advances in research of Helicobacter pylori detection methods. Hainan Medical Journal, 2020, 31(6):784-788.(in Chinese)

[23] 林美梅.幽门螺杆菌粪便抗原检测与尿素呼气试验对比研究.中国卫生标准管理, 2018, 9(18):119-122. Lin MM. Comparative study of Helicobacter pylori stool antigen test card andurea breath test. China Health Standard Management, 2018, 9(18):119-122.(in Chinese)

[24] Gisbert JP, de la Morena F, Abraira V. Accuracy of monoclonal stool antigen test for the diagnosis of H. pylori infection:a systematic review and meta-analysis. The American Journal of Gastroenterology, 2006, 101(8):1921-1930.

[25] Okuda M, Osaki T, Kikuchi S, Ueda J, Lin YS, Yonezawa H, Maekawa K, Hojo F, Kamiya S, Fukuda Y. Evaluation of a stool antigen test using a MAb for native catalase for diagnosis of Helicobacter pylori infection in children and adults. Journal of medical Microbiology, 2014, 63(Pt12):1621-1625.

[26] 何艳明,梁卓夫,姚淑雯,刘云锋,李彩金,周珍文. 3种方法对儿童幽门螺杆菌感染检测的比较.国际检验医学杂志, 2016, 37(2):145-146. He YM, Liang ZF, Yao SW, Liu YF, Li CJ, Zhou ZW. Comparison of three methods for the detection of Helicobacter pylori infection in children. International Journal of Laboratory Medicine, 2016, 37(2):145-146.(in Chinese)

[27] 王佐妤,谢立群,刘彩红,赵红艳.粪便抗原检测临床诊断幽门螺杆菌感染的可靠性研究.胃肠病学, 2015, 20(2):78-80. Wang ZY, Xie LQ, Liu CH, Zhao HY. Reliability of stool antigen test for clinical diagnosis of Helicobacter pylori infection. Chinese Journal of Gastroenterology, 2015, 20(2):78-80.(in Chinese)

[28] Alzoubi H, Al-Mnayyis A, Al rfoa I, Aqel A, Abu-Lubad M, Hamdan O, Jaber K. The use of ¹³C-urea breath test for non-invasive diagnosis of Helicobacter pylori infection in comparison to endoscopy and stool antigen test. Diagnostics, 2020, 10(7):448.

[29] Patel SK, Mishra GN, Pratap CB, Jain AK, Nath G. Helicobacter pylori is not eradicated after triple therapy:a nested PCR based study. BioMed Research International, 2014, 2014:483136.

[30] 林美梅.幽门螺旋杆菌的抗原检测与血清抗体检测的对比研究.中国卫生标准管理, 2019, 10(24):103-106. Lin MM. Comparative study of Helicobacter pylori stool antigen test card and serum HP antibody colloidal gold test. China Health Standard Management, 2019, 10(24):103-106.(in Chinese)

[31] 王小众,李丹.幽门螺杆菌的血清学诊断. 世界华人消化杂志, 2000, 8(5):550-551. Wang XZ, Li D. Serological diagnosis of Helicobacter pylori. World Chinese Journal of Digestology, 2000, 8(5):550-551.(in Chinese)

[32] Doohan D, Miftahussurur M, Matsuo Y, Kido Y, Akada J, Matsuhisa T, Yee TT, Htet K, Aftab H, Vilaichone RK, Mahachai V, Ratanachu-Ek T, Tshering L, Waskito LA, Fauzia KA, Uchida T, Syam AF, Rezkitha YAA, Yamaoka Y. Characterization of a novel Helicobacter pylori East Asian-type CagA ELISA for detecting patients infected with various cagA genotypes. Medical Microbiology and Immunology, 2020, 209(1):29-40.

[33] Leal YA, Flores LL, García-Cortés LB, Cedillo-Rivera R, Torres J. Antibody-based detection tests for the diagnosis of Helicobacter pylori infection in children:a meta-analysis. PLoS ONE, 2008, 3(11):e3751.

[34] Gong YH, Li QP, Yuan Y. Accuracy of testing for anti-Helicobacter pylori IgG in urine for H.pylori infection diagnosis:a systematic review and meta-analysis. BMJ Open, 2017, 7(4):e013248.

[35] 王龙,曹杰,辛毅.幽门螺杆菌抗体血清学检测的临床应用价值.国际消化病杂志, 2018, 38(6):390-392. Wang L, Cao J, Xin Y. Clinical value of serological detection of Helicobacter polori antibody. International Journal of Digestive Diseases, 2018, 38(6):390-392.(in Chinese)

[36] 谢庆,陈卫刚,齐翠花,刘芳,乔琨.胃蛋白酶原联合幽门螺旋杆菌IgG抗体对胃癌早期诊断的价值.胃肠病学和肝病学杂志, 2016, 25(11):1244-1247. Xie Q, Chen WG, Qi CH, Liu F, Qiao K. Value of serum pepsinogen and anti-H. pylori IgG in early diagnosis of gastric cancer. Chinese Journal of Gastroenterology and Hepatology, 2016, 25(11):1244-1247.(in Chinese)

[37] 刘文忠,谢勇,陆红,成虹,曾志荣,周丽雅,陈烨,王江滨,杜奕奇,吕农华.第五次全国幽门螺杆菌感染处理共识报告.胃肠病学, 2017, 22(6):346-360. Liu WZ, Xie Y, Lu H, Cheng H, Zeng ZR, Zhou LY, Chen Y, Wang JB, Du YQ, Lv NH. Fifth Chinese national consensus report on the management of Helicobacter pylori infection. Chinese Journal of Gastroenterology,2017, 22(6):346-360.(in Chinese)

[38] Hoshina S, Kahn SM, Jian W, Green PHR, Neu HC, Chin N, Morotomi M, LoGerfo P, Weinstein IB. Direct detection and amplification of Helicobacter pylori ribosomal 16S gene segments from gastric endoscopic biopsies. Diagnostic Microbiology and Infectious Disease, 1990, 13(6):473-479.

[39] Roth DE, Velapatiño B, Gilman RH, Su WW, Berg DE, Cabrera L, Garcia E. A comparison of a string test-PCR assay and a stool antigen immunoassay (HpSA) for Helicobacter pylori screening in peru. Transactions of the Royal Society of Tropical Medicine and Hygiene, 2001, 95(4):398-399.

[40] Gill P, Alvandi AH, Abdul-Tehrani H, Sadeghizadeh M. Colorimetric detection of Helicobacter pylori DNA using isothermal helicase-dependent amplification and gold nanoparticle probes. Diagnostic Microbiology and Infectious Disease, 2008, 62(2):119-124.

[41] Saez J, Belda S, Santibáñez M, Rodríguez JC, Sola-Vera J, Galiana A, Ruiz-García M, Brotons A, López-Girona E, Girona E, Sillero C, Royo G. Real-time PCR for diagnosing Helicobacter pylori infection in patients with upper gastrointestinal bleeding:comparison with other classical diagnostic methods. Journal of Clinical Microbiology, 2012, 50(10):3233-3237.

[42] Li CF, Ha TZ, Ferguson DA, Chi DS, Zhao RG, Patel NR, Krishnaswamy G, Thomas E. A newly developed PCR assay of H. pylori in gastric biopsy, saliva, and feces. Digestive Diseases and Sciences, 1996, 41(11):2142-2149.

[43] 迟文静,刘涛,刘宜昕,赵虎,张艳梅.幽门螺杆菌无创性分子生物学检测方法研究进展.检验医学, 2021, 36(8):880-885. Chi WJ, Liu T, Liu YX, Zhao H, Zhang YM. Research progress of non-invasive molecular biological methods for detecting Helicobacter pylori. Laboratory Medicine, 2021, 36(8):880-885.(in Chinese)

[44] Anand PS, Kamath KP, Anil S. Role of dental plaque, saliva and periodontal disease in Helicobacter pylori infection. World Journal of Gastroenterology, 2014, 20(19):5639-5653.

[45] Ogaya Y, Nomura R, Watanabe Y, Nakano K. Detection of Helicobacter pylori DNA in inflamed dental pulp specimens from Japanese children and adolescents. Journal of Medical Microbiology, 2015, 64(Pt 1):117-123.

[46] Amirhooshang A, Ramin A, Ehsan A, Mansour R, Shahram B. High frequency of Helicobacter pylori DNA in drinking water in Kermanshah, Iran, during June-November 2012. Journal of Water and Health, 2014, 12(3):504-512.

[47] 郗颖.幽门螺杆菌检测采用细菌培养与尿素酶试验的临床分析.临床医学研究与实践, 2016, 1(15):53. Xi Y. Clinical analysis of Helicobacter pylori detection by bacterial culture and urease test. Clinical Research and Practice, 2016, 1(15):53.(in Chinese)

[48] Gong YN, Li YM, Yang NM, Li HZ, Guo F, Lin L, Wang QY, Zhang JK, Ji ZZ, Mao JB, Mao JL, Shi ZC, Tang WH, Zhu XJ, Shao W, Zhang XF, Wang XH, Tong YF, Jiang MZ, Chen GL, Wang ZY, Tu HM, Jiang GF, Wu JS, Chen XP, Ding QL, Ouyang H, Jin FZ, Xu YL, Zhang JZ. Centralized isolation of Helicobacter pylori from multiple centers and transport condition influences. World Journal of Gastroenterology, 2015, 21(3):944-952.

[49] Toyoshima O, Nishizawa T, Koike K. Endoscopic Kyoto classification of Helicobacter pylori infection and gastric cancer risk diagnosis. World Journal of Gastroenterology, 2020, 26(5):466-477.

[50] Hernández-Garcés HR, Castellanos-González VV, González-Fabián L, Infante-Velázquez M, Peña K, Andrain-Sierra Y. Chromoendoscopy with red phenol in the diagnosis of Helicobacter pylori infection. Revista Española De Enfermedades Digestivas, 2012, 104(1):4-9.

[51] Gonen C, Simsek I, Sarioglu S, Akpinar H. Comparison of high resolution magnifying endoscopy and standard videoendoscopy for the diagnosis of Helicobacter pylori gastritis in routine clinical practice:a prospective study. Helicobacter, 2009, 14(1):12-21.

[52] Uedo N, Ishihara R, Iishi H, Yamamoto S, Yamamoto S, Yamada T, Imanaka K, Takeuchi Y, Higashino K, Ishiguro S, Tatsuta M. A new method of diagnosing gastric intestinal metaplasia:narrow-band imaging with magnifying endoscopy. Endoscopy, 2006, 38(8):819-824.

[53] Hoffman A, Goetz M, Vieth M, Galle PR, Neurath MF, Kiesslich R. Confocal laser endomicroscopy:technical status and current indications. Endoscopy, 2006, 38(12):1275-1283.

[54] Van Ijzendoorn MC, Laheij RJF, De Boer WA, Jansen JBMJ. The importance of corpus biopsies for the determination of Helicobacter pylori infection. The Netherlands Journal of Medicine, 2005, 63(4):141-145.

[55] Hartman DJ, Owens SR. Are routine ancillary stains required to diagnose Helicobacter infection in gastric biopsy specimens?An institutional quality assurance review. American Journal of Clinical Pathology, 2012, 137(2):255-260.

[56] Zúñiga-Noriega JR, Bosques-Padilla FJ, Pérez-Pérez GI, Tijerina-Menchaca R, Flores-Gutiérrez JP, Maldonado Garza HJ, Garza-González E. Diagnostic utility of invasive tests and serology for the diagnosis of Helicobacter pylori infection in different clinical presentations. Archives of Medical Research, 2006, 37(1):123-128.

[57] 范建华,吴瑾.快速尿素酶和组织病理学检测幽门螺杆菌的价值分析.药物生物技术, 2021, 28(1):26-29. Fan JH, Wu J. Value analysis on rapid urease test and histopathological detection of Helicobacter pylori. Pharmaceutical Biotechnology, 2021, 28(1):26-29.(in Chinese)

[58] Gravina AG, Zagari RM, De Musis C, Romano L, Loguercio C, Romano M. Helicobacter pylori and extragastric diseases:a review. World Journal of Gastroenterology, 2018, 24(29):3204-3221.

[59] Yang J, Dai LX, Pan X, Wang HR, Li B, Zhu J, Li MY, Shi XL, Wang BN. Protection against Helicobacter pylori infection in BALB/c mice by oral administration of multi-epitope vaccine of CTB-UreI-UreB. Pathogens and Disease, 2015, 73(5):DOI:10.1093/femspd/ftv026.

[60] Quaglia NC, Dambrosio A, Normanno G, Parisi A, Patrono R, Ranieri G, Rella A, Celano GV. High occurrence of Helicobacter pylori in raw goat, sheep and cow milk inferred by glmM gene:a risk of food-borne infection?International Journal of Food Microbiology, 2008, 124(1):43-47.

[61] Dore MP, Sepulveda AR, El-Zimaity H, Yamaoka Y, Osato MS, Mototsugu K, Nieddu AM, Realdi G, Graham DY. Isolation of Helicobacter pylori from sheep-implications for transmission to humans. The American Journal of Gastroenterology, 2001, 96(5):1396-1401.

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LI Yaqian, LIU Jiaqi, ZHU Xiaofang, DUAN Qiangde, ZHU Guoqiang. Research progress of detection technology of Helicobacter pylori and beyond. [J]. Acta Microbiologica Sinica, 2022, 62(5): 1645-1655

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Received:October 23,2021
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