Correlation between Akkermania and pulmonary edema of plateau cattle

Abstract:

[Objective] To investigate the relationship between Akkermansia and pulmonary edema of plateau cattle through the analysis of the gastrointestinal microbiota structure in plateau cattle. [Methods] The fecal samples were collected from healthy Jersey cattle in Shenyang (control), healthy Jersey cattle which had been introduced into Lhasa for half a year, local healthy yellow cattle in Lhasa, and Jersey cattle which had been introduced into Lhasa for six months and suffered from pulmonary edema. Illumina MiSeq was used for sequencing the V3–V4 region of the 16S rRNA gene in the samples. Microbiota structure and abundance were compared among the four fecal samples, thereby elucidating the correlation between Akkermansia and the pulmonary edema. [Results] The content of Akkermansia in the gastrointestinal tract of healthy local Lhasa yellow cattle was significantly higher than that of healthy Jersey cattle which had been introduced into Lhasa for half a year, and the content in Jersey cattle with pulmonary edema was significantly higher than that in healthy Jersey cattle that had been introduced into Lhasa for six months. Specifically, the abundance of Akkermansia in the gastrointestinal microbiota of healthy Jersey cattle from Shenyang, healthy Jersey cattle which had been introduced into Lhasa for half a year, local yellow cattle in Lhasa, and Jersey cattle which had been introduced into Lhasa for half a year with pulmonary edema was 0.07%, 0.09%, 6.62% (dominant genus), and 11.85% (the first dominant genus), respectively. [Conclusion] This paper investigated the relationship between Akkermansia and pulmonary edema of plateau cattle for the first time by Illumina MiSeq, which may provide a reference for using the abundance of Akkermansia as an indicator for the diagnosis of pulmonary edema, although the specific abundance value needs to be further determined.

Key words:Akkermansia;high-throughput sequencing;Lhasa plateau cattle;Jersey cattle;pulmonary edema