The two-component system EnvZ/OmpR mediates alkaline stress tolerance of Vibrio parahaemolyticus

doi:10.13343/j.cnki.wsxb.20220249

Home > Archive>Volume 62, Issue 12, 2022 >5043-5055. DOI:10.13343/j.cnki.wsxb.20220249

The two-component system EnvZ/OmpR mediates alkaline stress tolerance of Vibrio parahaemolyticus
DOI:
                        10.13343/j.cnki.wsxb.20220249
                    
CSTR:
                        32112.14.j.AMS.20220249
                    
Author:
                        YAO NingYAO Ning
Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Zhejiang Provincial Engineering Laboratory for Animal Health Inspection&Internet Technology, College of Animal Science and Technology, College of Veterinary Medicine of Zhejiang A&F University, Hangzhou 311300, Zhejiang, China
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LU ZhongLU Zhong
Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Zhejiang Provincial Engineering Laboratory for Animal Health Inspection&Internet Technology, College of Animal Science and Technology, College of Veterinary Medicine of Zhejiang A&F University, Hangzhou 311300, Zhejiang, China
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WANG FeiWANG Fei
Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Zhejiang Provincial Engineering Laboratory for Animal Health Inspection&Internet Technology, College of Animal Science and Technology, College of Veterinary Medicine of Zhejiang A&F University, Hangzhou 311300, Zhejiang, China
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ZHONG XiaojunZHONG Xiaojun
Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Zhejiang Provincial Engineering Laboratory for Animal Health Inspection&Internet Technology, College of Animal Science and Technology, College of Veterinary Medicine of Zhejiang A&F University, Hangzhou 311300, Zhejiang, China
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YANG MenghuaYANG Menghua
Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Zhejiang Provincial Engineering Laboratory for Animal Health Inspection&Internet Technology, College of Animal Science and Technology, College of Veterinary Medicine of Zhejiang A&F University, Hangzhou 311300, Zhejiang, China
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[Objective] Vibrio parahaemolyticus is a significant foodborne pathogen that causes economic and public health problems worldwide. In this study, we explored the roles of the two-component system (TCS) EnvZ/OmpR in mediating the alkaline stress tolerance of V.parahaemolyticus.[Methods] We identified the two-component system EnvZ/OmpR in the V.parahaemolyticus chromosomes with SMART (https://smart.embl.de/). Then, we constructed the envZ-deleted strain, the ompR-deleted strain, as well as the complemented strains. On this basis, we compared the alkaline stress tolerance of the wild-type strain and the mutants, and the mechanisms whereby the EnvZ/OmpR regulated the alkaline stress tolerance of V.parahaemolyticus were explored by qRT-PCR and bioluminescence reporter assay. [Results] Homology analysis of the VP0155 and VP0154 proteins revealed that their amino acid sequences shared a high identity with the two-component system EnvZ/OmpR. ompR deletion decreased the tolerance of V.parahaemolyticus to osmotic stress and alkaline stress, while the growth status of C△ompR, △envZ, and C△envZ was similar to that of the wild type. The transcription levels of porin genes vp1218, vp0493, vpa1745, vpa0085, and vpa1308 in △ompR were lower than those in the wild type, and the deletion strains of the porin genes (except for △vpa1308) showed low growth ability in the alkaline-shock environment as compared with the wild strains. In addition, OmpR inhibited the expression of AphB and △aphB was significantly more tolerant to alkaline stress than the wild type. Moreover, AphB can directly repress the transcription of vp0493 and vpa0085. [Conclusion] The two-component system EnvZ/OmpR mediates alkaline stress tolerance of V. parahaemolyticus, which depends on the transcriptional factor AphB to regulate the expression of the porins.

Key words:Vibrio parahaemolyticus;two-component system;EnvZ/OmpR;alkaline stress tolerance

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YAO Ning, LU Zhong, WANG Fei, ZHONG Xiaojun, YANG Menghua. The two-component system EnvZ/OmpR mediates alkaline stress tolerance of Vibrio parahaemolyticus. [J]. Acta Microbiologica Sinica, 2022, 62(12): 5043-5055

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Received:April 07,2022
Revised:May 07,2022
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Online: December 08,2022
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