Oxytetracycline residue alters profiles of microbial communities and metabolites during vegetable fermentation
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    Abstract:

    [Objective] To study the changes in microbial communities and metabolites during the vegetable fermentation exposed to oxytetracycline residue and further provide a theoretical basis for evaluating the effects of antibiotic residue on vegetable fermentation. [Methods] In this study, we determined the oxytetracycline residue by ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS), organic acids by high performance liquid chromatography (HPLC), volatile components by the electronic nose and gas chromatography mass spectrometry (GC-MS), microbial species by high-throughput sequencing. [Results] The oxytetracycline residue decreased from 4.00 mg/L to 2.53 mg/L in spontaneous vegetable fermentation. The vegetable without oxytetracycline residue experienced homolatic and heterolactic fermentation, while that with oxytetracycline residue only experienced homolatic fermentation. Meantime, the characteristic microorganisms changed from Lab. pentosus and Lab. plantarum to L. paratarrginis, Lab. buchneri, and Lab.kisonensis. Furthermore, oxytetracycline residue significantly affected the content of lactic acid, citric acid, acetic acid, citronellol, 3-octanol, allylsenevol, geranyl acetate, and phenylethyl isothiocyanate. [Conclusion] Oxytetracycline residue affected the type of vegetable lactic acid fermentation, the succession of microbial communities, and the production of organic acids and volatile compounds. Therefore, the antibiotic residue should be listed as a quality control indicator of raw vegetable materials for fermentation.

    Reference
    [1] PAN M, CHU LM. Fate of antibiotics in soil and their uptake by edible crops[J]. Science of the Total Environment, 2017, 599/600:500-512.
    [2] SHAHID M, KHAN MS. Tolerance of pesticides and antibiotics among beneficial soil microbes recovered from contaminated rhizosphere of edible crops[J]. Current Research in Microbial Sciences, 2021, 3:100091.
    [3] PAN M, CHU LM. Transfer of antibiotics from wastewater or animal manure to soil and edible crops[J]. Environmental Pollution, 2017, 231:829-836.
    [4] PROSSER RS, SIBLEY PK. Human health risk assessment of pharmaceuticals and personal care products in plant tissue due to biosolids and manure amendments, and wastewater irrigation[J]. Environment International, 2015, 75:223-233.
    [5] ZHI D, YANG DX, ZHENG YX, YANG Y, HE YZ, LUO L, ZHOU YY. Current progress in the adsorption, transport and biodegradation of antibiotics in soil[J]. Journal of Environmental Management, 2019, 251:109598.
    [6] ANAL AK, PERPETUINI G, PETCHKONGKAEW A, TAN R, AVALLONE S, TOFALO R, NGUYEN HV, CHU-KY S, HO PH, PHAN TT, WACHÉ Y. Food safety risks in traditional fermented food from South-East Asia[J]. Food Control, 2020, 109:106922.
    [7] XIAO YS, XIONG T, PENG Z, LIU CG, HUANG T, YU H, XIE MY. Correlation between microbiota and flavours in fermentation of Chinese Sichuan Paocai[J]. Food Research International, 2018, 114:123-132.
    [8] XIANG WL, ZHANG ND, LU Y, ZHAO QH, XU Q, RAO Y, LIU L, ZHANG Q. Effect of Weissella cibaria co-inoculation on the quality of Sichuan pickle fermented by Lactobacillus plantarum[J]. LWT, 2020, 121:108975.
    [9] RAO Y, QIAN Y, TAO YF, SHE X, LI YL, CHEN X, GUO SY, XIANG WL, LIU L, DU HJ, XIAO H. Characterization of the microbial communities and their correlations with chemical profiles in assorted vegetable Sichuan pickles[J]. Food Control, 2020, 113:107174.
    [10] 林凯, 蔡婷, 徐顾榕, 宋菲菲, 袁春红, 陈功, 向文良, 张庆. 有机白萝卜表皮附生乳酸菌抗生素耐药性分析[J]. 食品科学, 2015, 36(11):145-149. LIN K, CAI T, XU GR, SONG FF, YUAN CH, CHEN G, XIANG WL, ZHANG Q. Antibiotic resistance of epibiotic lactic acid bacteria on the surface of organic white radish[J]. Food Science, 2015, 36(11):145-149 (in Chinese).
    [11] LIU DQ, ZHANG CC, ZHANG JM, XIN XT, LIAO XJ. Metagenomics reveals the formation mechanism of flavor metabolites during the spontaneous fermentation of potherb mustard (Brassica juncea var. multiceps)[J]. Food Research International, 2021, 148:110622.
    [12] LIU XH, LV Y, XU K, XIAO XX, XI BD, LU SY. Response of ginger growth to a tetracycline-contaminated environment and residues of antibiotic and antibiotic resistance genes[J]. Chemosphere, 2018, 201:137-143.
    [13] 李佳楠. 城市污水系统中典型抗生素、抗性基因和抗性细菌的丰度与关联性研究[D]. 杭州:浙江大学硕士学位论文, 2014. LI JN. The abundance and relationships between typical antibiotics and corresponding resistance in municipal wastewater treatment system[D]. Hangzhou:Master's Thesis of Zhejiang University, 2014 (in Chinese)
    [14] LI ZJ, QI WN, FENG Y, LIU YW, EBRAHIM S, LONG J. Degradation mechanisms of oxytetracycline in the environment[J]. Journal of Integrative Agriculture, 2019, 18(9):1953-1960.
    [15] HALLING-SØRENSEN B, SENGELØV G, TJØRNELUND J. Toxicity of tetracyclines and tetracycline degradation products to environmentally relevant bacteria, including selected tetracycline-resistant bacteria[J]. Archives of Environmental Contamination and Toxicology, 2002, 42(3):263-271.
    [16] WATTS EG, JANES ME, PRINYAWIWATKUL W, SHEN YX, XU ZM, JOHNSON D. Microbiological changes and their impact on quality characteristics of red hot chilli pepper mash during natural fermentation[J]. International Journal of Food Science & Technology, 2018, 53(8):1816-1823.
    [17] CHEN ZD, KANG JY, ZHANG Y, YI XX, PANG XN, LI-BYARLAY H, GAO XZ. Differences in the bacterial profiles and physicochemical between natural and inoculated fermentation of vege?慡敢牬潥扳椠捦?摯敭渠楓瑨牡楮晸楩挠慐瑲楯潶湩?扣楥潛晊楝氮流?獮祡獬瑳攠浯?攠硍灩潣獲敯摢?瑯潬?瑧敹琬爠愲挰礲挰氬椠渷攰嬨?崩???焱甲愮挼畢汲琾畛爱攸??至え?ぉ???水??????????扅牅?孍??崠?剁?佋?奓??儠??乎?奙??吠?佁?奋???匬???塅?????奃???????婩????????????啮?????渭晶汯畬敡湴捩敬?漠晣?潭硰祯杵敮湤?攠硯灦漠獭畯牤敥?漠湫?晭散牨浩攠湴瑨慲瑯極潧湨?灦牥潲捭敥獮獴?慴湩摯?猠敢湹猠潬牡祣?煩畣愠污楣瑩楤攠獢?潣晴?卲楩捡桛畊慝渮?灌楗捔欬氠攲‰?倹愬漠挱愰椵?嬱?崸??刲匶???摲瘾慛渱挹敝猠??休ぎ??????????????さ?????ぉ??戠牙?嬬㈠?嵉??啙伬?千??????儠?????乢???????啣?塳女??偮唠????周桥攠?慲牡潤浩慴?捯潮浡灬漠獃楨瑩楮潥湳?漠晌?扺慨扯祵?杦楬湡杶敯牲?偬慩潱捵慯楲嬠?嵥???潮畴牡湴慩汯?漠晰??潣潥摳?儠畡慳氠楥瑶祡???ぴ?????????ㄠ???A profiles[J]. World Journal of Microbiology and Biotechnology, 2013, 29(3):559-567.
    [20] CAO JL, YANG JX, HOU QC, XU HY, ZHENG Y, ZHANG HP, ZHANG LB. Assessment of bacterial profiles in aged, home-made Sichuan Paocai brine with varying titratable acidity by PacBio SMRT sequencing technology[J]. Food Control, 2017, 78:14-23.
    [21] THIERRY A, POGAČIĆ T, WEBER M, LORTAL S. Production of flavor compounds by lactic acid bacteria in fermented foods[A]//Biotechnology of Lactic Acid Bacteria. Chichester[M].UK:John Wiley & Sons, Ltd, 2015:314-340.
    [22] SAWADA K, KOYANO H, YAMAMOTO N, YAMADA T. The relationships between microbiota and the amino acids and organic acids in commercial vegetable pickle fermented in rice-bran beds[J]. Scientific Reports, 2021, 11:1791.
    [23] EINSON JE, RANI A, YOU XM, RODRIGUEZ AA, RANDELL CL, BARNABA T, MAMMEL MK, KOTEWICZ ML, ELKINS CA, SELA DA. A vegetable fermentation facility hosts distinct microbiomes reflecting the production environment[J]. Applied and Environmental Microbiology, 2018, 84(22):e01680-e01618.
    [24] PASOLLI E, de FILIPPIS F, MAURIELLO IE, CUMBO F, WALSH AM, LEECH J, COTTER PD, SEGATA N, ERCOLINI D. Large-scale genome-wide analysis links lactic acid bacteria from food with the gut microbiome[J]. Nature Communications, 2020, 11:2610.
    [25] GAUDIN V. The growing interest in development of innovative optical aptasensors for the detection of antimicrobial residues in food products[J]. Biosensors, 2020, 10(3):21.
    [26] Yang XH, Song XF, Hallerman E, Huang ZT. Microbial community structure and nitrogen removal responses of an
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CAI Ting, XIONG Haibo, ZHOU Xiaohong, XIANG Wenliang, ZHANG Dafeng, PAN Maoyuan. Oxytetracycline residue alters profiles of microbial communities and metabolites during vegetable fermentation. [J]. Acta Microbiologica Sinica, 2023, 63(1): 206-219

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History
  • Received:April 14,2022
  • Revised:June 30,2022
  • Online: January 13,2023
  • Published: January 04,2023
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