The thioredoxin Lmo1903 contributes to oxidative stress tolerance of Listeria monocytogenes
Author:
  • CHEN Yuji

    CHEN Yuji

    Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, China-Australia Joint Laboratory for Animal Health Big Data Analytics, Zhejiang Provincial Engineering Research Center for Animal Health Diagnostics & Advanced Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, College of Animal Science and Technology/College of Veterinary Medicine, Zhejiang A&F University, Hangzhou 311300, Zhejiang, China
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  • Lü Xiaoling

    Lü Xiaoling

    Technical Center of Ningbo Customs, Ningbo Academy of Quarantine & Inspection Science and Technology, Ningbo 315100, Zhejiang, China
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  • HAN Yue

    HAN Yue

    Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, China-Australia Joint Laboratory for Animal Health Big Data Analytics, Zhejiang Provincial Engineering Research Center for Animal Health Diagnostics & Advanced Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, College of Animal Science and Technology/College of Veterinary Medicine, Zhejiang A&F University, Hangzhou 311300, Zhejiang, China
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  • LUO Ping

    LUO Ping

    Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, China-Australia Joint Laboratory for Animal Health Big Data Analytics, Zhejiang Provincial Engineering Research Center for Animal Health Diagnostics & Advanced Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, College of Animal Science and Technology/College of Veterinary Medicine, Zhejiang A&F University, Hangzhou 311300, Zhejiang, China
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  • CAO Qiyu

    CAO Qiyu

    Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, China-Australia Joint Laboratory for Animal Health Big Data Analytics, Zhejiang Provincial Engineering Research Center for Animal Health Diagnostics & Advanced Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, College of Animal Science and Technology/College of Veterinary Medicine, Zhejiang A&F University, Hangzhou 311300, Zhejiang, China
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  • CHEN Xindan

    CHEN Xindan

    Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, China-Australia Joint Laboratory for Animal Health Big Data Analytics, Zhejiang Provincial Engineering Research Center for Animal Health Diagnostics & Advanced Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, College of Animal Science and Technology/College of Veterinary Medicine, Zhejiang A&F University, Hangzhou 311300, Zhejiang, China
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  • WU Yukang

    WU Yukang

    Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, China-Australia Joint Laboratory for Animal Health Big Data Analytics, Zhejiang Provincial Engineering Research Center for Animal Health Diagnostics & Advanced Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, College of Animal Science and Technology/College of Veterinary Medicine, Zhejiang A&F University, Hangzhou 311300, Zhejiang, China
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  • XU Jiali

    XU Jiali

    Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, China-Australia Joint Laboratory for Animal Health Big Data Analytics, Zhejiang Provincial Engineering Research Center for Animal Health Diagnostics & Advanced Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, College of Animal Science and Technology/College of Veterinary Medicine, Zhejiang A&F University, Hangzhou 311300, Zhejiang, China
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  • GUAN Chiyu

    GUAN Chiyu

    Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, China-Australia Joint Laboratory for Animal Health Big Data Analytics, Zhejiang Provincial Engineering Research Center for Animal Health Diagnostics & Advanced Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, College of Animal Science and Technology/College of Veterinary Medicine, Zhejiang A&F University, Hangzhou 311300, Zhejiang, China
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  • SONG Houhui

    SONG Houhui

    Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, China-Australia Joint Laboratory for Animal Health Big Data Analytics, Zhejiang Provincial Engineering Research Center for Animal Health Diagnostics & Advanced Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, College of Animal Science and Technology/College of Veterinary Medicine, Zhejiang A&F University, Hangzhou 311300, Zhejiang, China
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  • CHENG Changyong

    CHENG Changyong

    Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, China-Australia Joint Laboratory for Animal Health Big Data Analytics, Zhejiang Provincial Engineering Research Center for Animal Health Diagnostics & Advanced Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, College of Animal Science and Technology/College of Veterinary Medicine, Zhejiang A&F University, Hangzhou 311300, Zhejiang, China
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    Abstract:

    [Objective] The thioredoxin family plays a key role in the oxidative stress response of the foodborne bacterial pathogen Listeria monocytogenes (LM) during environmental adaptation. Here, we studied the biological role of the thioredoxin Lmo1903 in oxidative stress tolerance.[Methods] The phylogenetic relationship and key active sites of Lmo1903 were analyzed by bioinformatics tools. The recombinant Lmo1903 protein was expressed and purified. The oxidoreductase activity of the recombination protein was determined with insulin as the substrate and the cellular localization was predicted after preparation of mouse polyclonal antibody with the recombinant protein. The oligonucleotide-directed site-specific deletion of cysteine from Lmo1903 protein was carried out to analyze the key sites of enzyme activity. The lmo1903-deleted strain Δlmo1903 was constructed by homologous recombination, and the complementation strain CΔlmo1903was constructed with the integrated complement plasmid pIMK2. The growth, motility, and oxidative stress tolerance of the strains were examined in vitro. [Results] Lmo1903 had a classical CX1X2C motif and was close related to the thioredoxin family member TrxA from Bacillus subtilis. It was mainly located in the cytoplasm of bacteria and possessed strong reductase activity. Cysteine was the key site for the enzymatic activity of Lmo1903. Deletion of lmo1903 did not affect bacterial growth, while it significantly weakened the tolerance to oxidative stress in Cu2+ stress environment. Furthermore, the deletion of this gene affected the transcriptional levels of the genes involved in bacterial flagellar formation and reduced the swimming motility. [Conclusion] The thioredoxin family member Lmo1903 exhibiting the reductase activity contributes to the oxidative stress tolerance and motility of L. monocytogenes.

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CHEN Yuji, Lü Xiaoling, HAN Yue, LUO Ping, CAO Qiyu, CHEN Xindan, WU Yukang, XU Jiali, GUAN Chiyu, SONG Houhui, CHENG Changyong. The thioredoxin Lmo1903 contributes to oxidative stress tolerance of Listeria monocytogenes. [J]. Acta Microbiologica Sinica, 2023, 63(3): 1269-1282

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History
  • Received:August 02,2022
  • Adopted:December 06,2022
  • Online: March 08,2023
  • Published: March 04,2023
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