Whole-genome sequencing reveals genotypic differences and potential probiotic properties of two Paocai-derived Lactiplantibacillus plantarum strains

Abstract:

[Objective] To explore the genotypic differences and potential probiotic properties of Lactiplantibacillus plantarum by using whole genome sequencing technology. [Methods] Based on the HiSeq and PacBio, this study sequenced the potential probiotic strains L. plantarum Eden-Star PC06 and L. plantarum Eden-Star PC108 with good evaluation of in vitro probiotic properties isolated from Sichuan multi-generation Paocai in the previous study. The relevant bioinformatics software was used to assemble the raw data and analyze the subsequent functional annotation, molecular evolution, strain safety, secondary metabolite synthesis gene clusters, and probiotic-related genes. [Results] The whole-genome information of two L. plantarum strains was obtained through gene assembly, and the genome sizes of L. plantarum Eden-Star PC06 and Eden-Star PC108 were 3 163 902 bp and 3 205 054 bp, respectively. The GC content of Eden-Star PC06 and Eden-Star PC108 was 44.68% and 44.67%, respectively, and they contained 3 161 and 3 197 DNA coding sequences, respectively. The comparison results of functional gene database showed that the two strains were extensively annotated on genes such as carbohydrate utilization, amino acid utilization, and glycosyltransferase. We found four genes related to intestinal fluid tolerance, intact gene clusters related to bacteriocin synthesis of L. plantarum, and probiotic genes resistant to multiple stresses on the whole genome of two strains of L. plantarum in the comparison database. [Conclusion] This study explores the genotypic differences and potential probiotic properties of two L. plantarum strains Eden-Star PC06 and Eden-Star PC108 at the gene level by whole-genome sequencing, and it has proved that L. plantarum Eden-Star PC06 and Eden-Star PC108 are potential probiotic strains with promising applications. This study is expected to provide a genetic basis for screening out excellent probiotic strains and evaluating their probiotic properties.

Key words:Lactiplantibacillus plantarum;whole genome sequencing;comparative genomics;functional gene;bile salt hydrolase;bacteriocin