ZHANG Shuyao
Key Laboratory of Agricultural Microbiomics and Precision Application(Ministry of Agriculture and Rural Affairs), Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Key Laboratory of Agricultural Microbiome(Ministry of Agriculture and Rural Affairs), State Key Laboratory of Applied Microbiology Southern China, Guangdong Detection Center of Microbiology, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, Guangdong, ChinaWEN Xia
Key Laboratory of Agricultural Microbiomics and Precision Application(Ministry of Agriculture and Rural Affairs), Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Key Laboratory of Agricultural Microbiome(Ministry of Agriculture and Rural Affairs), State Key Laboratory of Applied Microbiology Southern China, Guangdong Detection Center of Microbiology, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, Guangdong, ChinaSU Aiting
Key Laboratory of Agricultural Microbiomics and Precision Application(Ministry of Agriculture and Rural Affairs), Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Key Laboratory of Agricultural Microbiome(Ministry of Agriculture and Rural Affairs), State Key Laboratory of Applied Microbiology Southern China, Guangdong Detection Center of Microbiology, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, Guangdong, ChinaHUANG Di
Key Laboratory of Agricultural Microbiomics and Precision Application(Ministry of Agriculture and Rural Affairs), Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Key Laboratory of Agricultural Microbiome(Ministry of Agriculture and Rural Affairs), State Key Laboratory of Applied Microbiology Southern China, Guangdong Detection Center of Microbiology, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, Guangdong, ChinaTAO Hongbing
Guangdong Demay Biological Technology Co., Ltd., Guangzhou 510663, Guangdong, ChinaCHEN Yiwen
Key Laboratory of Agricultural Microbiomics and Precision Application(Ministry of Agriculture and Rural Affairs), Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Key Laboratory of Agricultural Microbiome(Ministry of Agriculture and Rural Affairs), State Key Laboratory of Applied Microbiology Southern China, Guangdong Detection Center of Microbiology, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, Guangdong, ChinaXIE Xiaobao
Key Laboratory of Agricultural Microbiomics and Precision Application(Ministry of Agriculture and Rural Affairs), Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Key Laboratory of Agricultural Microbiome(Ministry of Agriculture and Rural Affairs), State Key Laboratory of Applied Microbiology Southern China, Guangdong Detection Center of Microbiology, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, Guangdong, China[Objective] To type and identify 29 strains of Burkholderia cepacia complex (Bcc) isolated from daily chemical products in 2020–2022, re-identify 6 isolates identified as Burkholderia lata from daily chemicals products before 2020, and reveal the antibiotic resistance of Burkholderia aenigmatica sp. nov. [Methods] Bcc isolates from daily chemical products were subjected to multilocus sequence typing (MLST). Seven housekeeping genes, atpD, gltB, gyrB, recA, lepA, phaC, and trpB, were amplified by PCR. The sequencing results were compared with the data in MLST database to obtain the accession number of each housekeeping gene and the sequence type of each strain. Multilocus sequence analysis (MLSA) was employed to construct a phylogenetic tree based on the nucleotide sequences of alleles in MLST. The resistance of Bcc to common preservatives (1,3-dimethylmethylol-5,5-dimethylhydantoin, kathon, sodium benzoate, and potassium sorbate) and antibiotics (ceftazidime, kanamycin, and tetracycline) were determined by the minimum inhibitory concentration (MIC) method. [Results] The 29 Bcc isolates were identified as 5 species (B. cenocepacia, B. contaminans, B. aenigmatica, B. vietnamiensis, and B. stabilis) and typed as 15 sequence types. Seven new alleles and seven new sequence types (ST2118, ST2120, ST2122, ST2127, ST2128, ST2129, and ST2130) were identified in this study. The six B. lata strains from the samples before 2020 were re-identified as B. aenigmatica sp. nov. Only one of the 11 B. aenigmatica isolates was resistant to ceftazidime, and nine and eight B. aenigmatica isolates were resistant to kanamycin and tetracycline, respectively. Kathon and 1,3-dimethylmethylol-5,5-dimethylhydantoin (DMDMH) within the maximum permissible concentrations can effectively inhibit the growth of B. aenigmatica. Nine B. aenigmatica strains showed resistance to sodium benzoate and potassium sorbate. [Conclusion] The identification of Bcc is complex and there are unknown alleles and sequence types. B. aenigmatica has become the main Bcc species contaminating daily chemical products. Most strains of B. aenigmatica are resistant to aminoglycosides and tetracyclines. Most B. aenigmatica isolates from the daily chemical products have resistance to sodium benzoate and potassium sorbate.
ZHANG Shuyao, WEN Xia, SU Aiting, HUANG Di, TAO Hongbing, CHEN Yiwen, XIE Xiaobao. Typing and identification of Burkholderia cepacia complex and drug resistance of Burkholderia aenigmatica from daily chemical products. [J]. Acta Microbiologica Sinica, 2023, 63(9): 3616-3627
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