Research progress in the expression of antimicrobial peptides in microbial systems by genetic engineering

Abstract:

In recent years,antimicrobial peptides (AMPs) are considered alternatives to antibiotics and have received increasing attention.AMPs have a broad antibacterial spectrum and extensive sources and are not prone to drug resistance.At present,AMPs are mainly produced with three methods:extraction from natural sources,chemical synthesis,and microbial expression via genetic engineering.The application of the former two methods is limited due to their complicated processes,high costs,and low yields.Microbial expression via genetic engineering is more economical,scientific,and effective than the above two methods.This article introduces and compares the various expression systems and summarizes the strategies for increasing the heterologous expression levels,with a view to providing theoretical support for large-scale production of AMPs with low costs.

Key words:antimicrobial peptides;expression system;genetic engineering method;microorganisms