HUANG Jun
Hunan Institute of Microbiology, Changsha 410009, Hunan, China;Agricultural Microbiology Application Engineering and Technology Research Center of Hunan Province, Changsha 410009, Hunan, China;National Collection of Plant-associated Microbes (Hunan), Changsha 410009, Hunan, ChinaTANG Ying
Hunan Institute of Microbiology, Changsha 410009, Hunan, China;Agricultural Microbiology Application Engineering and Technology Research Center of Hunan Province, Changsha 410009, Hunan, China;National Collection of Plant-associated Microbes (Hunan), Changsha 410009, Hunan, ChinaHUANG Binbin
Hunan Institute of Microbiology, Changsha 410009, Hunan, China;Agricultural Microbiology Application Engineering and Technology Research Center of Hunan Province, Changsha 410009, Hunan, China;National Collection of Plant-associated Microbes (Hunan), Changsha 410009, Hunan, ChinaLI Ye
Hunan Institute of Microbiology, Changsha 410009, Hunan, China;Agricultural Microbiology Application Engineering and Technology Research Center of Hunan Province, Changsha 410009, Hunan, China;National Collection of Plant-associated Microbes (Hunan), Changsha 410009, Hunan, ChinaZHANG Cuiyang
Hunan Institute of Microbiology, Changsha 410009, Hunan, China;Agricultural Microbiology Application Engineering and Technology Research Center of Hunan Province, Changsha 410009, Hunan, China;National Collection of Plant-associated Microbes (Hunan), Changsha 410009, Hunan, ChinaGUO Zhaohui
Hunan Institute of Microbiology, Changsha 410009, Hunan, China;Agricultural Microbiology Application Engineering and Technology Research Center of Hunan Province, Changsha 410009, Hunan, China;National Collection of Plant-associated Microbes (Hunan), Changsha 410009, Hunan, ChinaLIU Qingshu
Hunan Institute of Microbiology, Changsha 410009, Hunan, China;Agricultural Microbiology Application Engineering and Technology Research Center of Hunan Province, Changsha 410009, Hunan, China;National Collection of Plant-associated Microbes (Hunan), Changsha 410009, Hunan, China[Objective] To study the plant disease-inhibiting and growth-promoting effects and identify the antimicrobial components of Streptomyces levis L2. [Methods] Morphological features and the phylogenetic tree based on the 16S rRNA gene sequences were employed to identify the strain L2 isolated from the rhizosphere of drought-tolerant Echinochloa crusgalli. The antimicrobial components produced by the strain were identified by chromatography and high-resolution mass spectrometry. The whole genome of the strain was sequenced by Illumina in combination with Nanopore. antiSMASH was employed to search for the biosynthetic gene clusters. [Results] Streptomyces levis L2 and its fermentation broth inhibited the growth of Gram-positive bacteria and phytopathogenic fungi, and the strain produced a large transparent zone on the CAS (chrome azurol sulphonate) plate and could produce indole-3-acetic acid (IAA). The antimicrobial components of the strain showed the m/z of 537.102 0 [M+H]+ (calcd for C27H20O12, 537.103 5, 2.2×10–6) and 523.086 3 [M+H]+ (calcd for C26H18O12, 523.087 8, 2.9×10–6), which were consistent with the exact masses of α-rubromycins, β-rubromycins and γ-rubromycins, with the errors less than parts per 5 million. Their HPLC retention time were in agreement with that of standard rubromycins. The whole genome of L2 had a length of 8.8 Mb and carried 32 biosynthetic gene clusters for secondary metabolites including rubromycins. [Conclusion] S. levis L2 exhibited excellent plant disease-inhibiting and growth-promoting properties, thus could be further developed as biocontrol agents. It produces the antimicrobial components rubromycins and its biosynthetic gene clusters contained 6 more modification genes than the previously published gene clusters for rubromycins and several genes with unknown functions.
HUANG Jun, TANG Ying, HUANG Binbin, LI Ye, ZHANG Cuiyang, GUO Zhaohui, LIU Qingshu. Characterization of Streptomyces levis L2 with plant disease-inhibiting and growth-promoting effects. [J]. Acta Microbiologica Sinica, 2024, 64(12): 4859-4868
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