High light and nitrogen deprivation affect the response of Chlamydomonas reinhardtii to naphthenic acid stress
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1.Key Laboratory of Plant Biotechnology of Liaoning Province, School of Life Science, Liaoning Normal University, Dalian, Liaoning, China;2.Dalian Key Laboratory of Genetic Resources for Marine Shellfish, Key Laboratory of Conservation and Exploitation of Aquatic Germplasm Resource, Ministry of Agriculture and Rural Affairs, Liaoning Ocean and Fisheries Science Research Institute, Dalian, Liaoning, China

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This work was supported by the Basic Scientific Research Project of Education Department of Liaoning Province (LJKQZ20222365), the PhD Initiation Research Project of Liaoning Normal University (2022BSL009), the National Natural Science Foundation of China (32402984), the Special Funding for Basic Research Operating Expenses of Undergraduate Universities in Liaoning Province (LS2024Q004), and the Horizontal Science and Technology Project of Liaoning Normal University (H2022011).

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    Abstract:

    Objective To explore the physiochemical response mechanism of a model algal strain Chlamydomonas reinhardtii that hyper-accumulates oils to exposure of naphthenic acids (NAs).Methods The impacts of a typical NA, cyclohexanecarboxylic acid (CHCA), on the physiochemical parameters, including growth, photosynthetic activity, pH value of the culture, uptake of nitrogen and phosphorus, and biochemical constituents (lipids, carbohydrates, proteins, and pigments), of C. reinhardtii with high initial cell density under high light-nitrogen repletion (HL+N) and high light-nitrogen deprivation (HL-N) conditions were studied.Results The exposure to CHCA prominently promoted the uptake of phosphorus by C. reinhardtii under HL+N, while significantly inceasing the relative abundance of saturated C16:0 and decreasing the relative abundance of polyunsaturated C18:3n3. In contrast, CHCA treatment significantly inhibited the photosynthetic activity and phosphorus uptake but did not affect the fatty acid profile of C. reinhardtii under HL-N stress. In addition, the growth, pH value of the culture, and content of lipids, carbohydrates, proteins, and pigments, in C. reinhardtii under both HL+N and HL-N conditions all remained relatively constant when subjected to CHCA exposure.Conclusion The tolerance of microalgae to environmental stress can be visualized by growth curves, photosythetic activity, uptake of nitrogen and phosphorus, and key biochemical constituents. HL+N improves the tolerance of C. reinhardtii to NAs by promoting the uptake of phosphorus and altering the fatty acid profile, while the case was contrary under HL-N. These findings are beneficial for establishing strategies on effective cultivation of microalgae that highly tolerate NAs.

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YANG Miao, LEI Hengping, YANG Ziyi, WU Meng, WEI Shiqi, XIE Xi, GONG Zheng. High light and nitrogen deprivation affect the response of Chlamydomonas reinhardtii to naphthenic acid stress. [J]. Acta Microbiologica Sinica, 2025, 65(5): 2252-2266

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History
  • Received:October 08,2024
  • Online: April 30,2025
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