Heterotrophic Cultivation of Chlorella for Beer brewery wastewater treatment

Abstract:

Abstract: [Objective] To treat wastewater from beer breweries, we used the wastewater for the heterotrophic cultivation of Chlorella. [Methods] Using basal medium containing 10 g/L glucose?H2O we screened 5 Chlorella strains of which 2 strains with high specific growth rate and maximal biomass were used wastewater treatment. [Results] The two strains, Chlorella pyrenoidosa 15-2070 and Chlorella vulgaris 15-2075, were suitable for heterotrophic mass cultivation, and had similar results. For Chlorella pyrenoidosa 15-2070, 5.3 g/L biomass was achieved with basal medium containing 10 g/L glucose?H2O and beer wastewater. In addition, the highest removal efficiencies for CODcr (92.2 %), BOD5 (95.1 %), NO3-_N (98.5 %), NH4+-N (92.3 %) were achieved during the process. [Conclusion]Beer wastewater could be used as a major component of the medium for heterotrophic cultivation. Most of the harmful compounds in the wastewater could be removed during this process.

Key words:Keywords: Chlorella; heterotrophic cultivation; wastewater treatment