Characterization of a carbendazim-degrading Trichoderma sp. T2-2 and its application in bioremediation
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Supported by Natural Science Foundation of Jiangsu, China(BK2004325)and the research grant of Jiangsu Committee of Science and Technology (BE2005175)

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    Abstract:

    Abstract: [Objective] To obtain carbendazim-degrading microbial strains, and to use them for bioremediation of contaminated soil. [Methods] A carbendazim-degrading bacterium T2-2 was isolated from the screening of drug-tolerated mutants Trichoderma strains. High-pressure liquid chromatography-mass spectrometry (HPLC-MS) analysis showed the presence of the metabolites after shake incubation of the Trichoderma T2-2 at temperature 25℃, 200r/min in mineral salt medium that contained 100mg/L carbendazim. We prepared T2-2 bioremediation agents from crop straw through solid fermentation. By inoculating T2-2 in soil, we performed a bioremediation test of sterilized soil and original soil at 0.1mg/g dry soil of carbendazim concentration and 107cfu/g dry soil of inoculating amount. In addition, we also conducted a control effect experiment of T2-2 against fusarium wilt of cucumber. [Results] The metabolites detected by HPLC-MS were 2-aminobenzimidazole, benzimidazole, and 2-aminobenxinitrile in the culture filtrate after 2 days of incubation. Carbendazim and metabolites could no longer be detected through the High-pressure liquid chromatography (HPLC) analysis in the culture filtrate after 5 days of incubation. In the soil bioremediation test, carbendazim in the sterilized soil was degraded completely after 6 days of inoculation, whereas the process only needed 4 days in original soil. It showed crop straw could function as co-metabolic substrate and promote co-metabolism of T2-2 and indigenous microorganisms. Moreover, the efficiency of T2-2 against cucumber fusarium wilt might reach 81.7%, which is superior to chemical pesticide. [Conclusion] T2-2 could degrade carbendazim in soil and thus control plant disease.

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Liansheng Tian, Fei Chen. Characterization of a carbendazim-degrading Trichoderma sp. T2-2 and its application in bioremediation. [J]. Acta Microbiologica Sinica, 2009, 49(7): 925-930

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  • Received:December 28,2008
  • Revised:March 22,2009
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