Li Guo
Graduate School, Jilin Agriculture University, Changchun 130118, ChinaNa Feng
Institute of Veterinary Medicine, Academy of Military Medical Science, Changchun 130000, ChinaSongtao Yang
Institute of Veterinary Medicine, Academy of Military Medical Science, Changchun 130000, ChinaXijun Wang
National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, ChinaJinying Ge
National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, ChinaXianzhu Xia
Institute of Veterinary Medicine, Academy of Military Medical Science, Changchun 130000, ChinaZhigao Bu
National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, ChinaSupported by the National Key Program for Technology Research and Development of China Plan Grant (2006BAD06A09), the National Programs for High Technology Research and Development of China (2006AA02Z456, 2006AA10A204) and the Key Project of Chinese Natio
Abstract: [Objective] To establish a reverse genetic system of rabies virus for producing safe and efficient rabies vaccine. [Method] By reverse genetic and molecular cloning technique, we established two rabies virus rescue systems including (1) help plasmids expressing N, P and L protein, and (2) CMV/T7 and T7 promoter. [Results] Wild-type rERA-VC was rescued by both systems, and had the same growth kinetics as parental virus. The third generation virus could grow to high titer. [Conclusion] The established reverse genetic system for rescuing wild-type rERA-VC provides the possibility of producing safe and efficient rabies virus vaccines.
Li Guo, Na Feng, Songtao Yang, Xijun Wang, Jinying Ge, Xianzhu Xia, Zhigao Bu. Reverse genetic system for rabies virus vaccine Evelyn-Rokitnicki-Abelseth strain. [J]. Acta Microbiologica Sinica, 2009, 49(7): 949-954
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