Transcriptional profiles of intestinal pig epithelial cell jejenum 2 cultures infected by vesicular stomatitis virus

Abstract:

Abstract: [Objective] To better understand the host inflammatory responses, in particular inflammatory cytokines responses of Vesicular Stomatitis virus (VSV) infection, and the host-VSV interaction. [Methods] We used VSV Indian strain to infect the Intestinal Pig Epithelial Cell Jejenum (IPEC-J2) cell. Then we measured and analyzed the viral RNA by using real-time PCR. The transcript level of cytokines (IL-2, 6, 8, 10,12, IFN-α, IFN –γ, TNF-α, TGF-β and TLR3) were detected by real-time PCR. [Results] We found that the transcript levels of IL-6, IL-8, IL-12, TNF-α and TLR3 were increased obviously whereas TGF-β showed no significant difference. TPEC-J2 cell did not secrete IL-2, IL-10, IFN-α and IFN–γ. [Conclusion] The level of inflammatory response was increased when VSV infected TPEC-J2 cell.

Key words:Keywords: Vesicular stomatitis virus; cytokines; IPEC-J2 cell; Transcriptional profiles