Characterization of Bacillus thuringiensis spoIIID gene Mutantation

Abstract:

Abstract: [Objective] Construction and characterization of a spoIIID gene deletion mutant of Bacillus thuringiensis. [Methods] Scanning electron microscopy and spore formation analysis were used to detect the ability of sporulation and formation of crystal protein in both the mutant and the wild strain. SDS-PAGE analysis was used to detect the expression of crystal protein. [Results] Scanning electron microscopy and spore formation analysis showed that spores were hardly produced and the crystal existed in the spoIIID deletion strain. SDS-PAGE results showed that the expression of cry gene in the mutant was decreased in Luria-Bertani medium, but not affected obviously in Schaeffer’s sporulation medium (SSM). [Conclusion]This indicated that the spoIIID gene was one of the essential genes for the sporulation of Bacillus thuringiensis, and influenced the expression of crystal protein.