Effects of Pa-pex11 gene on penicillin production in Penicillium anrantiogriseum

Abstract:

Abstract: [Objective] To study the effect of Pa-pex11 gene on penicillin production in Pencillium anrantiogriseum. [Method] Based on the conserved domain of pex11 from Penicillium chrysogenum, we designed primers pex01 and pex02 to amplify Pa-pex11 gene from P. anrantiogriseum. The complete Pa-pex11 gene was cloned via thermal asymmetric interlaced PCR (Tail-PCR). In order to obtain tranformants with additional Pa-pex11 gene copy, ATMT (Agrobacterium tumefaciens mediated transformation) was used to transform P. anrantiogriseum. The copy number of Pa-pex11 was measured by quantitative PCR. Penicillin production of the transformant was analyzed by bioassays and the microbodies were observed by transmission electron microscope (TEM). [Results] ATMT was successfully applied in P.anrantiogriseum. Increasing the copy number of Pa-pex11 gene resulted in a 1.7-fold increase of pencillin production, and the electron microscope graph showed that the number of microbodies increased obviously. [Conclusion] Our results suggest that increasing the copy number of Pa-pex11 can increase the number of microbodies, and stimulate the penicillin production.

Key words:Keywords: Pa-pex11 gene; Agrobacterium tumefaciens mediated transformation (ATMT); penicillin; microbodies