Screening and identification of a fungal strain functioning in hydroxylation of puerarin

Abstract:

Abstract: [Objective] Puerarin was a Chinese traditional medicine. We isolated a fungal from soil which could convert puerarin to 3′-hydroxypuerarin. [Methods] Strains were cultivated on potato dextrose agar (PDA) medium. The biotransformation product was extracted with an organic solvent and detected on high performance liquid chromatography (HPLC). [Results] A total of 186 fungal strains were isolated from soil, and strain NT-01was determined to transform puerarin into 3′-hydroxypuerarin. The product was identified as 3′-hydroxypuerarin by MS and 1H-NMR and 13C-NMR. The morphology characteristic of strain NT-01 was essentially consistent with Gliocladium deliguescens. Phylogenic analysis on ITS sequences showed that strain NT-01 was clustered together with G.deliguescens and G.viride with high boostrap supporting, so that strain NT-01 was identified as Gliocladium sp. [Conclusion] Gliocladium strain NT-01 can convert puerarin to 3′-hydroxypuerarin.

Key words:Keywords: puerarin; 3′-hydroxypuerarin; biotransformation; Gliocladium sp.