An improved replica plating method and its application to the high-throughput selection of transformants
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Supported by the National Programs for High Technology Research and Development of China (2007AA091904), the China Postdoctoral Sci-ence Foundation (20070410854) and the Natural Science Foundation of Guangdong Province (7301731)

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    Abstract:

    [Objective] To make a simpler and highly efficient replica plating tool to avoid the limitations of the traditional method. [Methods] A number of bamboo-toothpicks was sheaved to form a bundle with diameter close to the plate diameter. The sheaved bamboo-toothpicks were used for replica-plating. [Results] This replica plating tool was successfully used for the screening of Saccahromyces cerevisiae transformants. [Conclusion] Compared with the traditional tool, this tool was simpler and highly efficient.

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Minjun Xu, Xiaoqun Tian, Shining Zhou. An improved replica plating method and its application to the high-throughput selection of transformants. [J]. Acta Microbiologica Sinica, 2008, 48(5): 657-660

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History
  • Received:November 12,2007
  • Revised:February 19,2008
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