Actinobacterial diversity of a sediment sample from Xiaoerkule Lake

Abstract:

[Objective] The aim of study was to investigate Actinobacterial diversity in Xiaoerkule salt lake, to lay a foundation for furthering to tap. [Methods] Actinobacterial diversity in this sediment from Xiaoerkule Lake was investigated by culture-independent method and phylogenetic analysis based on 16S rRNA gene sequences. Total DNA of sediment sample was extracted using SDS-CTAB method. The primers for the class Actinobacteria were used for actinobacterial 16S rRNA gene amplification and then a clone library was constructed for the sediment sample. [Results] Fifty-one clones screened from 160 clones on the basis of Hae III digestion patterns were sequenced, and their sequences were deposited in the GenBank. Clone sequences (52.9%) belonged to Acidimicrobidae and 5 suborders of Actinobacteridae. The other clone sequences (47.1%), which formed one large distinct clade in phylogenetic tree among phylum Actinobacteria, may represent one new suborder or new class. [Conclusion] There was abundant actinobacterial diversity in the sediment of Xiaoerkule Lake, and the result implied that there were large numbers of unknown actionobacterial groups here.