Risk Assessment for Environmental Release of Genetically Engineered Mi-croorganism m-CDS-1

Abstract:

Abstract: [Objective] To assess the environmental release risk of genetically engineered microorganism Sphingomonas sp. m-CDS-1. [Methods] Pesticides detection, plates counting, most probable number-PCR (MPN-PCR) and denaturing gra-dient gel electrophoresis (DGGE) were used to study the pesticides degrading effects, the decline dynamics of m-CDS-1, and the effects of release of m-CDS-1 on community structure of soil microorganisms. [Results] m-CDS-1 (1.01× 107CFU/g dry soil) could degrade both 10.71 mg/kg methyl parathion and 1.29 mg/kg carbofuran in 30 days. m-CDS-1 declined quickly with time, and could not be detected anymore in 30 days. Release of m-CDS-1 would not change the number of the cultivable microorganisms notably. The similarities of PCR-DGGE profiles between the pesti-cides applied treatments and the controls in 4, 11 and 30 days were 17.16 %, 49.81 % and 75.01 %, and the similarities between the m-CDS-1 released treatments and controls were 49.57 %, 38.3 % and 83.3 %, respectively. In 60 days, DGGE profiles of all treatments and controls had high similarities. [Conclusion] m-CDS-1 could degrade both pesticides re-markably in the field, and would not be the predominant population, and would not affect the community structure of soil microorganisms in the long term.

Key words:Keywords: genetically engineered microorganism m-CDS-1; environmental release; risk assessment; pesticides degrada-tion; most probable number-PCR (MPN-PCR); denaturing gradient gel electrophoresis (DGGE)