聚乙烯醇固定化酵母细胞制备CTP研究

Study on CTP Production from CMP by Beer Yeast Cell Immobilized in PVA

摘要:

以聚乙烯醇为固定化载体，固定化冷冻处理过的啤酒酵母细胞从CMP制备CTP；分别从胶的型号、浓度和固定化方法的优化等方面摸索了最适的固定化条件，固定化细胞在试验条件下连续发酵8次，转化率维持在85%～95%。同时，还对固定化细胞的稳定性进行试验研究，并用HPLC对产品进行了分离鉴定。

关键词:聚乙烯醇, 固定化细胞, CTP

Abstract:

With PVA as the carrier, the frozen beer yeast cells were immobilized for production of CTP from CMP. we explored the optimal condition of the immobilization from the aspects of the type, concentration of the PVA, and the immobilizing methods of cells In all 8 continuous batch of fermentation under the reactional condition of the immobilized cells, the conversion rate of CTP were maintained about 85%～95%. Moreever, the storage stability of immobilized cells were investigated, and the products was also isolated and identifided by HPLC.

Key words:polyvinyl alcohol（PVA）, immobilized cells, CTP