大肠杆菌色氨酸生物合成途径关键酶的调控

Regulation of Key Enzymes in Tryptophan Biosynthesis Pathway in Escherichia coli

摘要:

为了通过基因工程手段提高大肠杆菌色氨酸产量, 对色氨酸生物合成途径中的关键基因trpR、tnaA、aroG和trpED进行了改造。首先通过敲除trpR基因解除了基因组上色氨酸合成和转运关键酶受到的反馈阻遏调控, 进而又敲除了tnaA基因, 阻断了色氨酸的分解代谢。然后, 将色氨酸合成途径的关键酶aroGfbr和trpEDfbr基因串联表达, 以去除色氨酸生物合成途径的瓶颈。与对照MG1655相比, trpR基因单敲菌色氨酸浓度提高了10倍, 双敲菌色氨酸浓度提高了约20倍。pZE12-trpEDfbr转入双敲菌后色氨酸浓度提高到168 mg/L, 而将aroGfbr和trpEDfbr转入双敲菌后, 色氨酸浓度提高到820 mg/L。为构建色氨酸高产菌奠定了基础。

关键词:aroG和trpED共表达, trpR和tnaA双敲除, 色氨酸

Abstract:

To improve tryptophan production in Escherichia coli, key genes in the tryptophan biosynthesis pathway -aroG, trpED, trpR and tnaA were manipulated. TrpR gene was knocked out to eliminate the repression on the key genes controlling tryptophan biosynthesis and transportation on bacteria chromosome, and the tryptophan degradation was blocked by tnaA gene knockout. Then the bottleneck in tryptophan biosynthesis pathway was removed by co-expressing aroGfbr gene and trpEDfbr gene. Compared with the MG1655, the tryptophan production of trpR knockout and double-genes knockout strains was improved 10-folds and about 20-folds, respectively. After the trpEDfbr was expressed, the tryptophan production increased to 168 mg/L, and when the aroGfbr and trpEDfbr were co-expressed, the tryptophan production increased to 820 mg/L. This work laid the foundation for further construction of higher-efficient engineered strain for tryptophan production.

Key words:aroGfbr and trpEDfbr, co-expression, trpR and tnaA double knockout, tryptophan production