禽流感病毒NS1蛋白对细胞的影响

Effect of Influenza Virus NS1 Protein on Host Cell

摘要:

NS1蛋白为流感病毒非结构蛋白, 只在病毒侵入宿主细胞后产生。目前NS1蛋白对细胞整体水平上的作用仍不清楚, 为了解NS1蛋白在病毒感染细胞中的作用, 构建了重组质粒pCMV-myc-NS1并将其转染A549细胞, 利用双向电泳技术检测了受NS1蛋白调控的宿主蛋白, 以期从蛋白质组水平上研究禽流感病毒与宿主细胞间的相互作用。同时, 还检测了转染NS1对细胞增殖和细胞周期的影响。结果显示, NS1在细胞中的表达, 能够明显引起宿主细胞代谢的变化, 并通过阻滞细胞周期的正常进行而减缓细胞的增殖。

关键词:NS1蛋白, 宿主细胞, 双向电泳, 细胞增殖, 细胞周期

Abstract:

NS1 is a non-structural protein of the influenza A virus, which could only be expressed when cells are infected. The effect of NS1 protein on host cell is still not clear. To understand the role of NS1 protein in cell infection, recombinant plasmid pCMV-myc-NS1 was constructed, and then transfected into A549 cells. Two-dimensional electrophoresis was employed to analyze proteins regulated by NS1 that could reflect the interaction between influenza virus and host cells at the protein level. The influence of NS1 on cell proliferation and cell cycle was also studied. The result showed that not only could NS1 remarkably affect metabolism, but it could also slow down cell proliferation through blocking cell cycle.

Key words:NS1, host cell, two-dimensional electrophoresis, cell proliferation, cell cycle