奇异变形杆菌的L-氨基酸脱氢酶在大肠杆菌中的异源表达和鉴定

Heterologous Expression and Characterization of L-Amino Acid Deaminase from Proteus mirabilis in Escherichia coli

摘要:

本研究在大肠杆菌中表达了奇异变形杆菌的L-氨基酸脱氢酶, 并对产物苯丙酮酸进行了鉴定。奇异变形杆菌的L-氨基酸脱氢酶在大肠杆菌中得到了很好的表达, 具有酶活性, 并且发现其活性与细胞膜相关。L-氨基酸脱氢酶与细胞膜的密切相关性, 对酶活来说可能是必不可少的。

关键词:奇异变形杆菌, 脱氢酶, 苯丙氨酸, 苯丙酮酸, 苯乳酸

Abstract:

We expressed an L-amino acid deaminase (Pma) from Proteus mirabilis in Escherichia coli and characterized the kinetics of phenylpyruvic acid production. P. mirabilis Pma was well expressed in E. coli in an active state and was found to be associated with membranes. The association of Pma with cellular membranes is likely to be necessary for its enzymatic activity.

Key words:proteus mirabilis, deaminase, phenylalanine, phenylpyruvic acid, phenyllactic acid