利用还原剂预处理提高药物蛋白稳定性的研究

Stability Improvement of a Therapeutic Protein by Reducing Agent Pretreatment

摘要:

在不断发展的蛋白质生物制药工程中, 蛋白质的不稳定往往是人们主要关注的问题。本研究利用对蛋白质进行还原剂预处理的策略, 提高了具有潜在治疗活性的重组人胞外域CD83蛋白的稳定性。在生理条件下, 蛋白治疗产品往往变性, 形成聚集和沉淀, 并最终被降解。由此证明还原剂预处理可以有效改善蛋白质的稳定性。

关键词:降解, 蛋白稳定性, 人胞外域的CD83蛋白, 预处理还原剂

Abstract:

A major concern in developing protein-based biopharmaceuticals is protein instability. A strategy with the use of reducing agent pretreatment to improve protein stability was developed for recombinant hCD83ext (i.e. the extracellular domain of human CD83) with a potential therapeutic activity. Under physiological conditions, the therapeutic product tended to denature, form aggregates and precipitates, and eventually degrade. The reducing agent pretreatment was demonstrated to be effective in improving the protein stability.

Key words:degradation, protein stability, hCD83ext, reducing agent pretreatment