重组乙酰胆碱酯酶的表达及其分子进化研究进展

Expression and molecular evolution of recombinant acetylcholinesterase for detection of pesticide residues: a review

摘要:

乙酰胆碱酯酶 (Acetylcholinesterase，AChE) 是酶抑制法检测农药残留的核心试剂。但天然提取的AChE存在含量低、纯化困难、稳定性差等不足，因此，高性能的重组AChE制备成为研究学者关注的热点。文中综述了重组AChE的表达、和分子进化等方面的研究成果，并指出酶定向进化策略与表面展示技术结合是重组AChE活性改造的未来趋势。

关键词:农药残留，重组乙酰胆碱酯酶，酶抑制检测法

Abstract:

Acetylcholinesterase (AChE) plays a key role in the pesticide determination. However, the extraction of AChE from natural materials has the disadvantages of low yield, complex purification and poor stability. Therefore, the preparation of recombinant AChE with high performance becomes the hot topic of researchers in recent years. In this article we summarize the progress in the expression of recombinant AChE and the improvement of its analytical characteristic. Finally, we point out that the directed evolution strategy combined with surface display technology is the future trend on improving recombinant AChE activity.

Key words:pesticide residues, recombinant acetylcholinesterase, enzyme inhibition system