途径特异性调控因子介导的链霉菌来源天然产物的产量提升

Improvement of natural product production in Streptomyces by manipulating pathway-specific regulators

摘要:

链霉菌是活性天然产物的重要来源。基因组学研究揭示了链霉菌有巨大的生物合成潜力，平均每株菌拥有20–40个生物合成基因簇。在常规的实验室条件下，大多数链霉菌来源的天然产物产量较低，影响了对其进一步研究和产业化开发。由于链霉菌中天然产物的生物合成受到严格的调控，对于这些调控因子和调控网络的深入研究将有力地促进链霉菌来源的天然产物的发现和开发利用。文中主要综述了近5年来链霉菌来源天然产物生物合成中途径特异性调控因子的研究进展，重点介绍其在提高相应天然产物产量中的应用。

关键词:链霉菌，天然产物，调控因子，产量提升

Abstract:

Streptomyces are major sources of bioactive natural products. Genome sequencing reveals that Streptomyces have great biosynthetic potential, with an average of 20–40 biosynthetic gene clusters each strain. However, most natural products from Streptomyces are produced in low yields under regular laboratory cultivation conditions, which hamper their further study and drug development. The production of natural products in Streptomyces is controlled by the intricate regulation mechanisms. Manipulation of the regulatory systems that govern secondary metabolite production will strongly facilitate the discovery and development of natural products of Streptomyces origin. In this review, we summarize progresses in pathway-specific regulators from Streptomyces in the last five years and highlight their role in improving the yields of corresponding natural products.

Key words:Streptomyces, nature products, regulators, titer improvement